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Updated: Mar 13, 2026
08:35
Identification of Alternative Splicing and Polyadenylation in RNA-seq Data
Published on: June 24, 2021
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Psoriasis-Specific RNA Isoforms Identified by RNA-Seq Analysis of 173,446 Transcripts.
Sulev Kõks1, Maris Keermann2, Ene Reimann1
1Department of Pathophysiology, Centre of Translational Medicine, University of Tartu, Tartu, Estonia; Department of Reproductive Biology, Estonian University of Life Sciences, Tartu, Estonia.
Frontiers in Medicine
|October 25, 2016
Summary
This study reveals significant differences in RNA alternative splicing in psoriasis. New RNA variants were identified, offering insights into this complex skin disease.
Area of Science:
- Molecular Biology
- Genomics
- Dermatology
Background:
- Previous studies explored transcriptome changes in psoriasis using microarrays and RNA-seq.
- No prior research analyzed alternatively spliced transcript expression profiles in psoriasis.
Purpose of the Study:
- To identify potential RNA isoforms with disease-specific expression profiles in psoriasis.
- To investigate differential expression of RNA splicing variants in psoriatic skin.
Main Methods:
- RNA sequencing data from lesional psoriatic (LP), non-lesional psoriatic (NLP), and normal control (C) skin were analyzed.
- Differential expression of RNA splicing variants was assessed by comparing LP vs. NLP, LP vs. C, and NLP vs. C.
Main Results:
- Analysis of 173,446 RNA isoforms revealed approximately 9,000 differentially expressed transcripts.
- Several novel RNA variants were identified, including ETV3_3, which was downregulated in psoriatic skin.
- Disease-specific transcripts (S100A7A, IL36RN_4, IL36G_3) involved in immune response were identified.
Conclusions:
- Psoriasis exhibits significant alterations in the expression of RNA alternative isoforms.
- The identification of novel isoforms enhances understanding of the molecular mechanisms underlying psoriasis.
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