Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: Mar 13, 2026

Determination of High-affinity Antibody-antigen Binding Kinetics Using Four Biosensor Platforms

Determination of High-affinity Antibody-antigen Binding Kinetics Using Four Biosensor Platforms

Published on: April 17, 2017

Modelling random antibody adsorption and immunoassay activity.

D Mackey¹, E Kelly, R Nooney

¹School of Mathematical Sciences, Dublin Institute of Technology, Kevin Street, Dublin 8, Ireland.

Mathematical Biosciences and Engineering : MBE

|October 25, 2016

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Reactogenicity and immunogenicity following heterologous and homologous third dose COVID-19 vaccination in UK adolescents (Com-COV3): A randomised controlled non-inferiority trial.

The Journal of infection·2025

Same author

Medical Student Attitudes to Physician Assisted Death.

Irish medical journal·2024

Same author

A systematic review and meta-analysis of the clinimetric properties of the core outcome measurement instruments for clinical effectiveness trials of nutritional and metabolic interventions in critical illness (CONCISE).

Critical care (London, England)·2023

Same author

Reaction thermodynamics as a constraint on piscine steroidogenesis flux distributions.

Comparative biochemistry and physiology. Part A, Molecular & integrative physiology·2023

Same author

Abstracts of presentations to the Annual Meetings of the Canadian Association of General Surgeons Canadian Association of Thoracic Surgeons Canadian Hepato-Pancreato-Biliary Society Canadian Society of Surgical Oncology Canadian Society of Colon and Rectal Surgeons: Victoria, BC Sept. 10-13, 2009.

Canadian journal of surgery. Journal canadien de chirurgie·2022

Same author

Outcomes of free flap reconstructive surgery in head and neck cancer patients over 80-years old.

The British journal of oral & maxillofacial surgery·2021

Same journal

Modeling the impact of budget limitation on the screening and treatment pathway of HPV-induced precancerous cervical lesions.

Mathematical biosciences and engineering : MBE·2026

Same journal

Modeling the effects of trait-mediated dispersal on coexistence of two species: Competition and non-consumptive predator-prey.

Mathematical biosciences and engineering : MBE·2026

Same journal

A close look at the viral reduction rate in target cell limited models.

Mathematical biosciences and engineering : MBE·2026

Same journal

A stochastic agent-based model for simulating tumor-immune dynamics and evaluating therapeutic strategies.

Mathematical biosciences and engineering : MBE·2026

Same journal

Addressing domain shift via imbalance-aware domain adaptation in embryo development assessment.

Mathematical biosciences and engineering : MBE·2026

Same journal

Effect of drug resistance on an HIV epidemic in heterogeneous populations.

Mathematical biosciences and engineering : MBE·2026

See all related articles

This study introduces a mathematical model to optimize antibody concentration in immunoassays. The model predicts the ideal antibody coverage for maximum sensitivity and detection limits in diagnostic assays.

Area of Science:

Biotechnology
Biophysics
Immunology

Background:

Immunoassay sensitivity relies on optimal capture antibody concentration and orientation on solid surfaces.
Antibody immobilization affects antigen-binding site accessibility due to orientation and packing density.

Purpose of the Study:

To develop a mathematical model predicting correctly oriented antibody concentration during immobilization.
To determine optimal antibody surface coverage for maximizing assay sensitivity and limit of detection.

Main Methods:

Utilized random sequential adsorption (RSA) theory to model antibody deposition.
Analyzed the evolution of correctly oriented antibody concentration under varying conditions.

Main Results:

More Related Videos

Rapid Determination of Antibody-Antigen Affinity by Mass Photometry

Rapid Determination of Antibody-Antigen Affinity by Mass Photometry

Published on: February 8, 2021

Determining Binding Affinity KD of Radiolabeled Antibodies to Immobilized Antigens

Determining Binding Affinity KD of Radiolabeled Antibodies to Immobilized Antigens

Published on: June 23, 2022

Related Experiment Videos

Last Updated: Mar 13, 2026

Determination of High-affinity Antibody-antigen Binding Kinetics Using Four Biosensor Platforms

Determination of High-affinity Antibody-antigen Binding Kinetics Using Four Biosensor Platforms

Published on: April 17, 2017

Rapid Determination of Antibody-Antigen Affinity by Mass Photometry

Rapid Determination of Antibody-Antigen Affinity by Mass Photometry

Published on: February 8, 2021

Determining Binding Affinity KD of Radiolabeled Antibodies to Immobilized Antigens

Determining Binding Affinity KD of Radiolabeled Antibodies to Immobilized Antigens

Published on: June 23, 2022

The model predicts how antibody concentration and orientation change during immobilization.
Identified competing effects of high antibody concentration (denaturation vs. upright orientation).
Demonstrated the existence of an optimal surface coverage for maximum active antibody concentration.

Conclusions:

The developed mathematical model aids in optimizing antibody concentration for enhanced immunoassay performance.
Understanding antibody orientation and coverage is crucial for improving assay sensitivity and detection limits.