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Related Experiment Video

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Three-dimensional Optical-resolution Photoacoustic Microscopy
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Handheld optical-resolution photoacoustic microscopy.

Li Lin1, Pengfei Zhang1, Song Xu2

  • 1Washington University in St. Louis, Optical Imaging Laboratory, Department of Biomedical Engineering, One Brookings Drive, St. Louis, Missouri 63130, United States.

Journal of Biomedical Optics
|October 25, 2016
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Summary

Researchers created a compact handheld optical-resolution photoacoustic microscopy (OR-PAM) probe for fast, label-free in vivo imaging. This flexible OR-PAM system enables high-resolution visualization of microvasculature and skin lesions.

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Area of Science:

  • Biomedical optics
  • Medical imaging
  • Photoacoustics

Background:

  • Optical-resolution photoacoustic microscopy (OR-PAM) provides label-free in vivo imaging.
  • It achieves high spatial resolution by detecting optical absorption via the photoacoustic effect.

Purpose of the Study:

  • To develop a compact handheld OR-PAM probe for rapid photoacoustic imaging.
  • To enhance flexibility for imaging diverse anatomical locations.

Main Methods:

  • A compact, handheld OR-PAM probe was designed and constructed.
  • The probe incorporates a two-axis, water-immersible microelectromechanical system (MEMS) mirror for beam scanning.
  • System performance was evaluated through in vivo imaging in mouse ears and human volunteers.

Main Results:

  • The handheld probe demonstrated feasibility for fast photoacoustic imaging.
  • Successful in vivo imaging of capillary beds in mouse ears was achieved.
  • Imaging of capillary beds and a mole in a human volunteer confirmed system performance.

Conclusions:

  • The developed handheld OR-PAM probe offers a flexible and compact solution for in vivo photoacoustic imaging.
  • This technology facilitates high-resolution imaging of microvasculature and superficial structures.
  • The system shows potential for various clinical and research applications requiring portable imaging devices.