Conditions of radioiodination with iodogen as oxidizing agent

Different factors that give the maximum iodination of proteins by iodogen method have been evaluated. The quantity of iodogen was varied from 2 to 20 micrograms; pH from 3 to 9; the quantity of bovine serum albumin (BSA) from 25 micrograms to 2 mg; the 131I activity from 200 microCi to 5 mCi and the reaction time from 2 to 30 min. The maximum iodination of proteins is achieved with iodogen-to-iodide molar ratio of 100-200 and iodogen-to-protein molar ratio of 1-2 at pH 6-8 for 10-15 min at room temperature.