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Bone marrow-derived innate macrophages attenuate oxazolone-induced colitis
Zhao Cheng1, Hong-Ling Peng1, Rong Zhang2
1Department of Hematology, Institute of Molecular Hematology, The Second Xiang-ya Hospital, Central South University, Changsha, Hunan, PR China.
Cellular Immunology
|October 26, 2016
Summary
Bone marrow-derived innate macrophages (BMIMs) differentiated into M2 macrophages can reduce inflammation. Intravenous transplantation of these M2 macrophages alleviated oxazolone-induced colitis in a mouse model.
Area of Science:
- Immunology
- Cell Biology
Background:
- Granulocyte macrophage colony-stimulating factor (GM-CSF)-dependent F4/80high CD11bhigh innate macrophages can be derived from bone marrow cells in vitro.
- These innate macrophages can be differentiated into M1 or M2 subtypes.
Purpose of the Study:
- To investigate the potential of bone marrow cell-derived innate macrophages (BMIMs) to exert anti-inflammatory effects.
- To assess the efficacy of M2-polarized BMIMs (BMIM-M2Ms) following intravenous transplantation in a mouse model of Th2-mediated inflammation.
Main Methods:
- BMIMs were cultured in vitro and polarized into M2 macrophages using Interleukin-4 (IL-4) and Interleukin-13 (IL-13).
- M2 macrophages were intravenously transplanted into mice with oxazolone (OXZ)-induced colitis.
- The therapeutic effects were evaluated by assessing inflammation severity, colon length, body weight, and M2 macrophage infiltration.
Main Results:
- Transplantation of BMIM-M2Ms significantly attenuated intestinal inflammation in the OXZ-induced colitis model.
- Improved colon lengths and mouse body weights were observed in treated mice.
- Accumulation of F4/80+ CD206+ M2 macrophages was detected in the colons of transplanted mice.
Conclusions:
- Bone marrow-derived innate macrophages, when differentiated into M2 macrophages, demonstrate significant anti-inflammatory properties in vivo.
- BMIM-M2Ms show therapeutic potential for alleviating Th2-mediated inflammatory conditions like OXZ-induced colitis.
