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Cell Specific Gene Expression

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...

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Related Experiment Video

Updated: Mar 13, 2026

Cell Type-specific Gene Expression Profiling in the Mouse Liver

Cell Type-specific Gene Expression Profiling in the Mouse Liver

Published on: September 17, 2019

A Transcriptomic Signature of Mouse Liver Progenitor Cells.

Adam M Passman¹, Jasmine Low², Roslyn London³

¹School of Chemistry and Biochemistry, The University of Western Australia, Crawley, WA 6009, Australia; The Centre for Medical Research, Harry Perkins Institute of Medical Research, Nedlands, WA 6009, Australia.

Stem Cells International

|October 26, 2016

Summary

This summary is machine-generated.

Researchers identified novel gene markers to detect liver progenitor cells (LPCs) in diseased livers. This aids in diagnosing liver diseases and monitoring treatment effectiveness.

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Last Updated: Mar 13, 2026

Cell Type-specific Gene Expression Profiling in the Mouse Liver

Cell Type-specific Gene Expression Profiling in the Mouse Liver

Published on: September 17, 2019

Hepatic Progenitor Specification from Pluripotent Stem Cells using a Defined Differentiation System

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Isolation and Enrichment of Liver Progenitor Subsets Identified by a Novel Surface Marker Combination

Isolation and Enrichment of Liver Progenitor Subsets Identified by a Novel Surface Marker Combination

Published on: February 18, 2017

Area of Science:

Hepatology
Cancer Stem Cell Biology
Transcriptomics

Background:

Liver progenitor cells (LPCs) are crucial for liver regeneration but are also implicated in liver disease and hepatocellular carcinoma (HCC).
Accurate detection of LPCs is vital for diagnosing liver pathologies and monitoring treatment efficacy.
Current methods for LPC detection may lack sensitivity, speed, or reliability.

Purpose of the Study:

To identify novel molecular markers for the sensitive and reliable detection of LPCs in liver tissue.
To understand the biological pathways associated with LPCs, particularly in the context of liver disease and HCC.
To establish a transcriptomic signature that distinguishes LPCs from other liver cell types.

Main Methods:

A meta-analysis of over 400 microarrays comparing LPC lines with various stem cell and liver datasets (embryonic, developed liver, HCC).
Identification of gene clusters that differentiate LPCs from other liver cell types.
Validation of identified LPC markers using quantitative PCR (qPCR) and immunohistochemistry (IHC).

Main Results:

Three distinct gene clusters were identified, highlighting the proliferative nature of LPCs and their link to HCC.
Twenty-six novel LPC markers, including Mcm2 and Ltbp3, and eight known markers were discovered.
Validated markers accurately detected LPCs in pathological liver tissue and correlated with LPC abundance.

Conclusions:

Global transcript profiling is a valuable approach for identifying diagnostic and prognostic markers for liver diseases.
The identified LPC markers provide tools for improved diagnosis and monitoring of liver pathologies.
Understanding LPC biology is key to developing targeted therapies for liver diseases and HCC.