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Sequence of the gene for murine complement component C4.

R T Ogata1, P A Rosa, N E Zepf

  • 1Division of Molecular Biology, Medical Biology Institute, La Jolla, California 92037.

The Journal of Biological Chemistry
|October 5, 1989
PubMed
Summary
This summary is machine-generated.

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Researchers sequenced the murine complement component C4 gene, revealing its full 15,956 base pair length. The study details 40 introns, including repetitive elements and a novel sequence, offering insights into gene structure and evolution.

Area of Science:

  • Immunogenetics
  • Molecular Biology
  • Genomics

Background:

  • The murine complement component C4 (C4) gene is located within the S region of the major histocompatibility complex (H-2).
  • Understanding the complete nucleotide sequence of the C4 gene is crucial for studying its structure, function, and evolutionary relationship with human C4 genes.

Purpose of the Study:

  • To determine and report the complete nucleotide sequence of the murine complement component C4 gene.
  • To analyze the intron-exon structure and identify repetitive elements within the murine C4 gene.

Main Methods:

  • DNA sequencing of the murine C4 gene from a SmaI restriction site near the 5' end to a KpnI site 3' of the polyadenylation site.
  • Bioinformatic analysis to identify introns, exons, and repetitive DNA sequences.

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Main Results:

  • The complete nucleotide sequence of the murine C4 gene spans 15,956 base pairs.
  • The gene contains 40 introns ranging from 75 to 1089 base pairs, including murine B1 and MT repeat elements, and a novel repeat sequence.
  • An intron is precisely located at the site of C4 proteolytic activation by complement protease C1s.

Conclusions:

  • The intron-exon structure of the murine C4 gene has been fully elucidated.
  • The presence of repetitive elements and a novel sequence within introns provides insights into gene regulation and evolution.
  • Comparison with human C4 genes suggests the independent loss of a large intron in both species.