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A Quantitative Mass-Spectrometry Platform to Monitor Changes in Cysteine Reactivity
1Department of Chemistry, Boston College, 2609 Beacon Street, Chestnut Hill, MA, USA.
Abstract:
Cysteine residues on proteins serve diverse functional roles in catalysis and regulation and are susceptible to numerous posttranslational modifications. Methods to monitor the reactivity of cysteines within the context of a complex proteome have facilitated the identification and functional characterization of cysteine residues on disparate proteins. Here, we describe the use of a cysteine-reactive iodoacetamide probe coupled to isotopically labeled, cleavable linkers to identify and quantify cysteine-reactivity changes from two biological samples.
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