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Updated: Mar 13, 2026

High Throughput Danio Rerio Energy Expenditure Assay
Published on: January 27, 2016
Do method and species lifestyle affect measures of maximum metabolic rate in fishes?
S S Killen1, T Norin1, L G Halsey2
1Institute of Biodiversity, Animal Health and Comparative Medicine, Graham Kerr Building, University of Glasgow, Glasgow, G12 8QQ, U.K.
Abstract:
The rate at which active animals can expend energy is limited by their maximum aerobic metabolic rate (MMR). Two methods are commonly used to estimate MMR as oxygen uptake in fishes, namely during prolonged swimming or immediately following brief exhaustive exercise, but it is unclear whether they return different estimates of MMR or whether their effectiveness for estimating MMR varies among species with different lifestyles. A broad comparative analysis of MMR data from 121 fish species revealed little evidence of different results between the two methods, either for fishes in general or for species of benthic, benthopelagic or pelagic lifestyles.
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