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Controlled Microfluidic Environment for Dynamic Investigation of Red Blood Cell Aggregation

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Rotation-Driven Microfluidic Disc for White Blood Cell Enumeration Using Magnetic Bead Aggregation.

Yiwen Ouyang¹, Jingyi Li¹, Doris M Haverstick²

¹Department of Chemistry, University of Virginia , McCormick Road, P.O. Box 400319, Charlottesville, Virginia 22904, United States.

Analytical Chemistry

|October 26, 2016

Summary

A new low-cost microfluidic platform integrates a rotation-driven microdisc with a magnetic field for higher-throughput DNA and cell enumeration. This automated pinwheel assay detects down to single-cell DNA levels, correlating well with clinical results.

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Area of Science:

Biotechnology
Microfluidics
Assay Development

Background:

Previous magnetic bead assays for DNA enumeration were cost-efficient but labor-intensive and difficult to integrate.
Open-well formats required manual steps and lacked adaptability for automated systems.

Purpose of the Study:

To develop a low-cost, higher-throughput, and integrated microfluidic platform for DNA and cell enumeration.
To adapt magnetic bead-based agglutination assays for automation and multiplexing.

Main Methods:

A disposable rotation-driven microdisc (RDM) was fabricated using laser printing, cutting, and lamination.
A bidirectional rotating magnetic field (bi-RMF) was employed for simultaneous actuation of multiple assays on the RDM.
On-chip serial dilution was integrated for sample preparation within the microfluidic disc.

Main Results:

The integrated RDM and bi-RMF system enabled higher-throughput pinwheel assays.
The assay detected human genomic DNA down to 5.5 picograms, equivalent to the mass in a single cell.
Enumeration of white blood cells in human blood samples showed high correlation (C.V. of 10%) with clinical laboratory results.

Conclusions:

The developed RDM-based pinwheel assay offers a cost-effective (<$2) and automated solution for magnetic particle-based agglutination assays.
This platform demonstrates a promising strategy for automation and multiplexing in diagnostic applications.
The system achieves high sensitivity and accuracy comparable to established clinical methods.