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High-performance liquid chromatography of proteins on N-methylpyridinium polymer columns
A Sugii1, K Harada, K Nishimura
1Faculty of Pharmaceutical Sciences, Kumamoto University, Japan.
Journal of Chromatography
|June 23, 1989
Abstract:
Two types of 4-methylpyridinium polymers (4VP-DVB-Me and 4VP-EG-Me, cross-linked with divinylbenzene and ethylene glycol dimethacrylate, respectively) were employed for the analysis of proteins in ion-exchange high-performance liquid chromatography. These polymers had different physical properties in the dry state, but showed similar retentions in size-exclusion chromatography using carbohydrate standards. Generally, the 4VP-EG-Me column was superior to the 4VP-DVB-Me column with regard to separation and recovery of proteins.