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A Method of Targeted Cell Isolation via Glass Surface Functionalization
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Controlled Detachment of Chemically Glued Cells.

Heebeom Koo1,2, Sei Kwang Hahn3, Seok Hyun Yun1

  • 1Wellman Center for Photomedicine, Massachusetts General Hospital and Harvard Medical School , 65 Landsdowne Street, UP-5, Cambridge, Massachusetts 02139, United States.

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We developed a novel cell-glue system using metabolic glycoengineering and click chemistry for reversible cell adhesion. This system allows rapid, minimally cytotoxic cell gluing and detachment triggered by glutathione.

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Area of Science:

  • Biochemistry
  • Cell Biology
  • Materials Science

Background:

  • Cell-cell adhesion is crucial for biological processes.
  • Existing methods for artificial cell adhesion often lack reversibility or exhibit cytotoxicity.

Purpose of the Study:

  • To develop a chemically detachable cell-glue system.
  • To enable rapid and reversible control over artificial cell-cell adhesion.

Main Methods:

  • Metabolic glycoengineering to introduce azide groups on cell surfaces.
  • Bioorthogonal click chemistry (tetrazine-TCO) for cell conjugation.
  • Disulfide bond-containing linkers for chemical detachment.

Main Results:

  • Successfully created a cell-glue system using azide-modified and tetrazine (Tz) or trans-cyclooctene (TCO) modified cells.
  • Achieved rapid cell gluing (<10 min) via Tz-TCO click chemistry.
  • Demonstrated rapid (<10 min) and minimally cytotoxic detachment using glutathione to cleave disulfide bonds.

Conclusions:

  • The developed cell-glue system offers a rapid and reversible method for controlling artificial cell-cell adhesion.
  • This system holds potential for applications in tissue engineering and cell-based assays requiring dynamic cell assembly.