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New fluorescence assay for the quantitation of fungi
T Coleman1, J V Madassery, G S Kobayashi
1Infectious Diseases Division, Jewish Hospital, St. Louis, Missouri.
Journal of Clinical Microbiology
|September 1, 1989
Abstract:
Quantitative determination of fungal mass is easily achieved with a new procedure that detects particle epifluorescence. Fungi are detected after exposure to a fluorescent stain (Fungiqual; CIBA-GEIGY Corp., Summit, N.J.) by using a fluorescence particle concentration analyzer. This report describes a simple fluorescence method for quantitation of either yeast or mycelial forms of fungi. The nature of the staining reaction was studied, and a practical application of this procedure for determination of fungal susceptibility to an antifungal agent is presented.