Related Experiment Video

Updated: Mar 13, 2026

Modeling Oral-Esophageal Squamous Cell Carcinoma in 3D Organoids
10:43

Modeling Oral-Esophageal Squamous Cell Carcinoma in 3D Organoids

Published on: December 23, 2022

4.2K

Overexpression of SRC-3 promotes esophageal squamous cell carcinoma aggressiveness by enhancing cell growth and

Fang-Ping Xu1, Yan-Hui Liu1, Xin-Lan Luo1

  • 1Department of Pathology and Laboratory Medicine, Guangdong General Hospital & Guangdong Academy of Medical Sciences, Guangzhou, China.

Cancer Medicine
|October 27, 2016
PubMed

Insights

Steroid receptor coactivator-3 (SRC-3) is overexpressed in esophageal squamous cell carcinomas (ESCCs), correlating with poorer patient survival. Targeting SRC-3 may offer a new therapeutic strategy for ESCC.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cancer Research

Background:

  • Steroid receptor coactivator-3 (SRC-3) is a key transcriptional coactivator implicated in various cancers.
  • Limited research exists on SRC-3's role in esophageal squamous cell carcinomas (ESCCs), necessitating further investigation into its function in tumor progression.

Purpose of the Study:

  • To investigate the expression and functional significance of SRC-3 in human esophageal squamous cell carcinomas (ESCCs).
  • To determine if SRC-3 serves as a prognostic marker and potential therapeutic target in ESCC.

Main Methods:

  • Quantitative PCR (qPCR), Western blot, and immunohistochemistry were used to assess SRC-3 expression in ESCC cell lines and patient tissues.
  • Functional assays including proliferation, invasion, tumorigenicity, flow cytometry, and Chromatin Immunoprecipitation were performed to elucidate SRC-3's role in cancer progression.
  • Cox regression analysis was employed for prognostic evaluation.

Main Results:

  • SRC-3 was overexpressed in 48% of ESCC cases.
  • SRC-3 overexpression significantly correlated with poorer overall and progression-free survival in ESCC patients.
  • SRC-3 was identified as an independent prognostic marker for ESCC.
  • Activation of insulin-like growth factor (IGF)/AKT pathway was linked to SRC-3's influence on cell growth and invasiveness in ESCC cell lines.

Conclusions:

  • SRC-3 overexpression is clinically relevant and functionally contributes to the progression of human ESCC.
  • SRC-3 represents a potential molecular target for improving ESCC prognosis and treatment strategies.
Keywords:
Cell growthcell invasionesophageal squamous cell carcinomainsulin-like growth factor/AKTsteroid receptor coactivator-3

More Related Videos

Development of Compendium for Esophageal Squamous Cell Carcinoma03:36

Development of Compendium for Esophageal Squamous Cell Carcinoma

Published on: April 12, 2024

888
An Immunofluorescent Method for Characterization of Barrett’s Esophagus Cells08:54

An Immunofluorescent Method for Characterization of Barrett’s Esophagus Cells

Published on: July 20, 2014

14.5K

Related Concept Videos

Rous Sarcoma Virus (RSV) and Cancer01:03

Rous Sarcoma Virus (RSV) and Cancer

Rous Sarcoma virus or RSV was discovered by F. Peyton Rous in the year 1911 as a filterable transmissible agent that could cause tumors in chickens. He won a Nobel Prize for this discovery in 1966. His experiments clearly demonstrated that some cancers could be caused by infectious agents and led to the discovery of many more cancer-causing viruses in animals as well as humans.
RSV is a retrovirus that contains two copies of a plus-strand  RNA genome. Its genome consists of four main open...
6.5K
Rous Sarcoma Virus (RSV) and Cancer01:03

Rous Sarcoma Virus (RSV) and Cancer

5.9K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
5.4K
Induced Pluripotent Stem Cells01:06

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic...
5.9K
Mitogens and the Cell Cycle02:38

Mitogens and the Cell Cycle

Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...
8.3K
Tumor Progression02:07

Tumor Progression

Tumor progression is a phenomenon where the pre-formed tumor acquires successive mutations to become clinically more aggressive and malignant. In the 1950s, Foulds first described the stepwise progression of cancer cells through successive stages.
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...
7.7K