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Hyperpolarized 13C Metabolic Magnetic Resonance Spectroscopy and Imaging
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Microwave-gated dynamic nuclear polarization.

Aurélien Bornet1, Arthur Pinon1, Aditya Jhajharia2

  • 1Ecole Polytechnique Fédérale de Lausanne, Institut des Sciences et Ingénierie Chimiques, 1015 Lausanne, Switzerland.

Physical Chemistry Chemical Physics : PCCP
|October 27, 2016
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Summary
This summary is machine-generated.

Dissolution dynamic nuclear polarization (D-DNP) with microwave gating significantly boosts 13C polarization in nuclear magnetic resonance (NMR). This method achieves high 13C polarization rapidly, improving NMR signal enhancement.

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Area of Science:

  • Nuclear Magnetic Resonance Spectroscopy
  • Magnetic Resonance Imaging

Background:

  • Dissolution dynamic nuclear polarization (D-DNP) is a key technique for enhancing Nuclear Magnetic Resonance (NMR) signals.
  • Combining cross-polarization (CP) with D-DNP offers rapid, high 13C polarization.
  • Optimizing D-DNP protocols is crucial for maximizing NMR sensitivity.

Purpose of the Study:

  • To investigate the impact of microwave irradiation gating on CP-D-DNP efficiency.
  • To achieve significantly enhanced 13C polarization levels in short build-up times.
  • To demonstrate the effectiveness of this optimized D-DNP approach for NMR applications.

Main Methods:

  • Implementation of microwave gating, temporarily switching off microwave irradiation before CP.
  • Application of the CP-D-DNP technique with microwave gating to 13C-labeled compounds.
  • Measurement of 13C polarization levels and build-up time constants.

Main Results:

  • Switching off microwave irradiation prior to CP substantially increases polarization efficiency.
  • Achieved 13C polarization of up to 64% for sodium [1-13C]acetate with a 160 s build-up time.
  • Reached a 13C polarization of 78% for [13C]urea using the optimized method.

Conclusions:

  • Microwave gating is an effective strategy to boost CP-D-DNP performance.
  • This optimized D-DNP method enables rapid acquisition of highly polarized NMR signals.
  • The technique holds significant potential for advancing NMR sensitivity and applications.