Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Transgenic Plants02:50

Transgenic Plants

9.0K
Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
9.0K
Transgenic Organisms00:53

Transgenic Organisms

34.2K
Overview
34.2K
Plant Tissue Culture02:57

Plant Tissue Culture

41.1K
Plant tissue culture is widely used in both primary and applied science. Applications range from plant development studies to functional gene studies, crop improvement, commercial micropropagation, virus elimination, and conservation of rare species.
41.1K
Plant Breeding and Biotechnology01:59

Plant Breeding and Biotechnology

22.1K
Crop cultivation has a long history in human civilization, with records showing the cultivation of cereal plants beginning at around 8000 BC. This early plant breeding was developed primarily to provide a steady supply of food.
22.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A key residue of the extracellular gate provides quality control contributing to ABCG substrate specificity.

Nature communications·2025
Same author

The suberin transporter StABCG1 is required for barrier formation in potato leaves.

Scientific reports·2025
Same author

The secreted PAMP-induced peptide StPIP1_1 activates immune responses in potato.

Scientific reports·2023
Same author

An LRR receptor kinase controls ABC transporter substrate preferences during plant growth-defense decisions.

Current biology : CB·2023
Same author

Lysophosphatidylcholine 17:1 from the Leaf Surface of the Wild Potato Species <i>Solanum bulbocastanum</i> Inhibits <i>Phytophthora infestans</i>.

Journal of agricultural and food chemistry·2021
Same author

A phosphoinositide 5-phosphatase from Solanum tuberosum is activated by PAMP-treatment and may antagonize phosphatidylinositol 4,5-bisphosphate at Phytophthora infestans infection sites.

The New phytologist·2020

Related Experiment Video

Updated: Mar 13, 2026

Agroinfiltration and PVX Agroinfection in Potato and Nicotiana benthamiana
07:33

Agroinfiltration and PVX Agroinfection in Potato and Nicotiana benthamiana

Published on: January 3, 2014

21.4K

Potato plants with genetically engineered tropane alkaloid precursors.

Nadine Küster1, Sabine Rosahl2, Birgit Dräger3,4

  • 1Department of Pharmaceutical Biology, Martin-Luther University Halle-Wittenberg, Hoher Weg 8, 06120, Halle (Saale), Germany.

Planta
|October 27, 2016
PubMed
Summary

Tropinone reductase II (TRII) is crucial for calystegine biosynthesis in potato plants. Suppressing TRII significantly reduced calystegine accumulation, offering insights into alkaloid pathway functions.

Keywords:
CalystegineGenetic modificationPutrescine N-methyltransferaseSolanum tuberosumTropinone reductases

More Related Videos

Agrobacterium tumefaciens and Agrobacterium rhizogenes-Mediated Transformation of Potato and the Promoter Activity of a Suberin Gene by GUS Staining
08:31

Agrobacterium tumefaciens and Agrobacterium rhizogenes-Mediated Transformation of Potato and the Promoter Activity of a Suberin Gene by GUS Staining

Published on: March 29, 2019

30.9K
Potato Virus X-Based microRNA Silencing VbMS In Potato.
11:51

Potato Virus X-Based microRNA Silencing VbMS In Potato.

Published on: May 11, 2020

3.6K

Related Experiment Videos

Last Updated: Mar 13, 2026

Agroinfiltration and PVX Agroinfection in Potato and Nicotiana benthamiana
07:33

Agroinfiltration and PVX Agroinfection in Potato and Nicotiana benthamiana

Published on: January 3, 2014

21.4K
Agrobacterium tumefaciens and Agrobacterium rhizogenes-Mediated Transformation of Potato and the Promoter Activity of a Suberin Gene by GUS Staining
08:31

Agrobacterium tumefaciens and Agrobacterium rhizogenes-Mediated Transformation of Potato and the Promoter Activity of a Suberin Gene by GUS Staining

Published on: March 29, 2019

30.9K
Potato Virus X-Based microRNA Silencing VbMS In Potato.
11:51

Potato Virus X-Based microRNA Silencing VbMS In Potato.

Published on: May 11, 2020

3.6K

Area of Science:

  • Plant biochemistry
  • Alkaloid biosynthesis
  • Molecular genetics

Background:

  • Calystegines are hydroxylated alkaloids found in potato roots and tubers.
  • Their function in plants remains unclear, despite their in vitro inhibition of glycosidases.
  • The tropane alkaloid pathway is responsible for calystegine formation.

Purpose of the Study:

  • To investigate the roles of key enzymes in calystegine biosynthesis in Solanum tuberosum.
  • To elucidate the specific functions of putrescine N-methyltransferase (PMT) and tropinone reductases (TRI, TRII) in this pathway.

Main Methods:

  • Genetic manipulation of potato plants using RNA interference (RNAi) and overexpression.
  • Altering the activity of PMT, TRI, and TRII enzymes.
  • Quantifying calystegine accumulation and related intermediates in transformed plants.

Main Results:

  • Overexpression of putrescine N-methyltransferase (PMT) did not affect calystegine levels.
  • Solanum tuberosum tropinone reductase I (StTRI) activity was confirmed in vivo, but did not influence calystegine accumulation.
  • Suppression of StTRII via RNAi severely reduced calystegine formation in potato sprouts.

Conclusions:

  • StTRII plays a critical role in calystegine biosynthesis in potato.
  • StTRI is functional but not rate-limiting for calystegine production.
  • Calystegine-deprived potato plants (StTRII RNAi) provide a model for studying calystegine functions under natural conditions.