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Cementogenesis and osteogenesis in periodontal tissue regeneration by recombinant human transforming growth factor-β3
Ugo Ripamonti1, Ruqayya Parak1,2, Roland M Klar1,3
1Bone Research Laboratory, Department of Oral Medicine & Periodontology, School of Oral Health Sciences, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, Johannesburg, South Africa.
Objectives:
The aim of this study was to investigate cementogenesis and alveolar bone induction during in vivo periodontal tissue regeneration upon implantation of hTGF-β3 in furcation defects of Papio ursinus and to evaluate the feasibility of gene expression studies.
Materials And Methods:
Class II furcation defects (day 0) were prepared in mandibular first and second molars of three P. ursinus and on day 30 implanted with and without 75 μg hTGF-β3 in Matrigel® matrix. On day 0, 30 and 90, cementum and alveolar bone were harvested for gene expression analyses. Coral-derived bioreactors with and without 250 μg hTGF-β3 were implanted in the rectus abdominis to monitor tissue induction.
Results:
hTGF-β3 induced cementogenesis with TGF-β3 , Cementum Protein-1 (Cemp1) and Osteocalcin (OC) up-regulation, and down-regulation of BMP-2 and OP-1. Matrigel® matrix specimens showed up-regulation of BMP-2, TGF-β3 , and OC, with down-regulation of OP-1 and Cemp1. hTGF-β3 induced alveolar bone with down-regulation of OP-1, TGF-β3 , OC, and Cemp1. hTGF-β3 bioreactors induced bone at the periphery only. BMP-3, BMP-4, TGF-β1 and TGF-β3 were up-regulated in the adjacent muscle with TGF-β2 down-regulation.
Conclusions:
Cementogenesis and osteogenesis by hTGF-β3 entail the expression and up-regulation of TGF-β3 and OC with fine tuning and modulation of BMP-2 and OP-1.
