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Next generation sequencing and its applications in HPV-associated cancers
Musaffe Tuna1, Christopher I Amos1
1Department of Biomedical Data Science, Geisel School of Medicine, Dartmouth College, Lebanon.
Oncotarget
|October 27, 2016
Summary
Human papillomavirus (HPV) causes many cancers. Next-generation sequencing (NGS) reveals HPV integration sites and genetic changes, aiding personalized cancer treatment strategies.
Area of Science:
- Oncology
- Virology
- Genomics
Background:
- Approximately 18% of human cancers are virally linked, with human papillomavirus (HPV) being a primary cause of cervical, head and neck, anal, penile, and vulvar cancers.
- Advances in next-generation sequencing (NGS) and omics technologies have significantly enhanced understanding of HPV-driven tumor pathogenesis.
Purpose of the Study:
- To review the role of HPV integration sites and their functional consequences in cervical and head and neck cancers.
- To highlight the importance of integrating various omics and clinical data for personalized cancer treatment.
Main Methods:
- Review of current literature on HPV integration sites and associated genetic/epigenetic alterations.
- Analysis of data from next-generation sequencing (NGS) and other omics approaches.
Main Results:
- NGS and omics studies provide detailed insights into genomic HPV integration sites, disrupted genes, and pathways in HPV-associated cancers.
- Common and distinct genetic and epigenetic alterations are identified across different HPV-driven malignancies.
Conclusions:
- Understanding HPV integration sites and their functional impact is crucial for elucidating cancer pathophysiology.
- Integrating multi-omics and clinical data is essential for developing targeted and personalized therapies for HPV-driven cancers.
