Reduced uterine perfusion pressure T-helper 17 cells cause pathophysiology associated with preeclampsia during...

Preeclampsia is associated with chronic inflammation and an imbalance among T-helper cell subtypes with an increase in T-helper 17 (T_H17) cells. The objective of this study was to determine a role for T_H17s, from the reduced uterine perfusion pressure (RUPP) rat model of preeclampsia, in the etiology of hypertension and chronic inflammation during pregnancy. CD4⁺/CD25^- T cells were isolated from rat spleens, cultured in T_H17 media, and were verified as T_H17s via flow cytometry. On day 12 of gestation, 1×10⁶ T_H17 cells from RUPP rats were adoptively transferred into NP rats, carotid catheters were inserted on day 18, and on day 19, mean arterial pressure (MAP) was recorded, serum and plasma were collected, and oxidative stress and production of agonistic autoantibodies to the ANG II type I receptor (AT₁-AA) were analyzed. MAP increased from 100.3 ± 1.7 mmHg in normal pregnant (NP; n = 17) to 124.8 ± 2.1 mmHg in RUPP (n = 22; P < 0.0001) and to 110.8 ± 2.8 mmHg in NP+RUPP T_H17 (n = 11). Pup weights in NP+RUPP T_H17s were decreased to 1.92 ± 0.09 g from 2.39 ± 0.14 in NP rats (P < 0.01). AT₁-AA significantly increased from 0.1 ± 0.2 beats/min in NP to 15.6 ± 0.7 beats/min in NP+RUPP T_H17s. IL-6 was 22.3 ± 5.7 pg/ml in NP and increased to 60.45 ± 13.8 pg/ml in RUPP (P < 0.05) and 75.9 ± 6.8 pg/ml in NP+RUPP T_H17 rats (P < 0.01). Placental and renal oxidative stress were 238 ± 27.5 and 411 ± 129.9 relative light units·min^-1·mg^-1 in NP and 339 ± 104.6 and 833 ± 331.1 relative light units·min^-1·mg^-1 in NP+RUPP T_H17, respectively. In conclusion, RUPP T_H17 cells induced intrauterine growth restriction and increased blood pressure, AT₁-AA, IL-6, and tissue oxidative stress when transferred to NP rats, indicating a role for autoimmune associated T_H17 cells, to cause much of the pathophysiology associated with preeclampsia.