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Published on: March 8, 2018
Chloroplast sequence of treegourd (Crescentia cujete, Bignoniaceae) to study phylogeography and domestication
Priscila Ambrósio Moreira1, Cédric Mariac2, Nora Scarcelli2
1Post-Graduate Program in Botany, Instituto Nacional de Pesquisas da Amazônia (INPA), Av. André Araújo 2936, Petrópolis, 69067-375 Manaus, Amazonas, Brazil.
Premise Of The Study:
Crescentia cujete (Bignoniaceae) fruit rinds are traditionally used for storage vessels and handicrafts. We assembled its chloroplast genome and identified single-nucleotide polymorphisms (SNPs).
Methods And Results:
Using a genome skimming approach, the whole chloroplast of C. cujete was assembled using 3,106,928 sequence reads of 150 bp. The chloroplast is 154,662 bp in length, structurally divided into a large single copy region (84,788 bp), a small single copy region (18,299 bp), and two inverted repeat regions (51,575 bp) with 88 genes annotated. By resequencing the whole chloroplast, we identified 66 SNPs in C. cujete (N = 30) and 68 SNPs in C. amazonica (N = 6). Nucleotide diversity was estimated at 1.1 × 10-3 and 3.5 × 10-3 for C. cujete and C. amazonica, respectively.
Conclusions:
This broadened C. cujete genetic toolkit will be important to study the origin, domestication, diversity, and phylogeography of treegourds in the Neotropics.
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