Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Role Of Notch Signalling In Intestinal Stem Cell Renewal01:12

Role Of Notch Signalling In Intestinal Stem Cell Renewal

2.6K
Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
2.6K
Renewal of Intestinal Stem Cells01:23

Renewal of Intestinal Stem Cells

3.5K
The intestinal epithelial lining rapidly renews every 4 to 5 days. The renewal is facilitated by intestinal stem cells (ISCs) located at the base of the crypt– a gland located at the bottom of each villus. ISCs divide asymmetrically to form new stem cells and progenitor daughter cells. The daughter cells are called transit-amplifying (TA) cells which move upwards along the crypt and either differentiate into absorptive cells– the enterocytes or secretory cells– including the...
3.5K
Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal01:22

Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal

2.8K
Erythropoietin-producing hepatocellular carcinoma receptor (Eph) and its ligand, Eph receptor-interacting protein (Ephrin) were first discovered in the human carcinoma cell line, hence the name. Ephrin-Eph interaction guides cells to reach their appropriate location in adult tissues. They also play an essential role in the immune system by helping in immune cell migration, adhesion, and activation. Based on their structure and function, Eph is divided into two classes — EphA and EphB.
2.8K
Adult Stem Cells01:33

Adult Stem Cells

34.1K
Stem cells are undifferentiated cells that divide and produce more stem cells or progenitor cells that differentiate into mature, specialized cell types. All the cells in the body are generated from stem cells in the early embryo, but small populations of stem cells are also present in many adult tissues including the bone marrow, brain, skin, and gut. These adult stem cells typically produce the various cell types found in that tissue—to replace cells that are damaged or to continuously...
34.1K
Mitochondria01:37

Mitochondria

21.1K
Mitochondria are eukaryotic cellular organelles that are known to produce energy through a process called oxidative phosphorylation. Besides their primary function, mitochondria are involved in various cellular processes, including cell growth, differentiation, signaling, metabolism, and senescence. Age-related changes cause a decline in mitochondrial quality and integrity due to increased mitochondrial mutations and oxidative damage. Thus, aging can severely impact mitochondrial functions,...
21.1K
The Inner Mitochondrial Membrane01:28

The Inner Mitochondrial Membrane

4.9K
The inner mitochondrial membrane is the primary site of ATP synthesis. The inner membrane domain that forms a smooth layer adjacent to the outer membrane is called the inner boundary membrane. This domain contains membrane transporters that drive metabolites in and out of the mitochondria.  In contrast, the inner membrane network that invaginates into the matrix space is called the cristae membrane. This domain accounts for principle mitochondrial function as it accommodates the protein...
4.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Vancomycin eliminates gut deoxycholic acid, restoring ER proteostasis in ILC2s and relieving colitis.

JCI insight·2026
Same author

Corrigendum to Protein tyrosine phosphatase nonreceptor type 2 controls colorectal cancer development.

The Journal of clinical investigation·2026
Same author

Atypical Ductular Reactions Are a Distinct Regenerative Phenomenon in Patients With Hepatocellular Carcinoma (HCC) After Transarterial Chemoembolization.

Laboratory investigation; a journal of technical methods and pathology·2026
Same author

Author Correction: Cleavage of roquin and regnase-1 by the paracaspase MALT1 releases their cooperatively repressed targets to promote T<sub>H</sub>17 differentiation.

Nature immunology·2026
Same author

Targeting the tumour's Achilles heel: ATR inhibition to exploit a constitutive vulnerability of hepatoblastoma.

Journal of hepatology·2026
Same author

A Novel Murine Model of Hemophagocytic Lymphohistiocytosis-Like Inflammation in ZNFX1 Deficiency.

European journal of immunology·2026
Same journal

Large-scale discovery and annotation of substructure patterns in mass spectrometry profiles.

Nature communications·2026
Same journal

Salmonella SopB suppresses post-transcriptionally regulated cytokine release to reduce early tissue inflammation and delay disease progression.

Nature communications·2026
Same journal

A human-specific microRNA controls the timing of excitatory synaptogenesis.

Nature communications·2026
Same journal

An HMA-like integrated domain in the wheat tandem kinase WTK4 recognises an RNase-like pathogen effector.

Nature communications·2026
Same journal

Learning regularities in noise engages both neural predictive activity and representational changes.

Nature communications·2026
Same journal

The H3K4 methyltransferase KMT2D is an essential cofactor for GATA1 at erythroid gene enhancers.

Nature communications·2026
See all related articles

Related Experiment Video

Updated: Mar 13, 2026

Real Time Analysis of Metabolic Profile in Ex Vivo Mouse Intestinal Crypt Organoid Cultures
08:53

Real Time Analysis of Metabolic Profile in Ex Vivo Mouse Intestinal Crypt Organoid Cultures

Published on: November 3, 2014

16.7K

Mitochondrial function controls intestinal epithelial stemness and proliferation.

Emanuel Berger1, Eva Rath1, Detian Yuan2

  • 1Technische Universität München, Chair of Nutrition and Immunology, 85350 Freising-Weihenstephan, Germany.

Nature Communications
|October 28, 2016
PubMed
Summary
This summary is machine-generated.

Mitochondrial function is vital for intestinal stemness. Loss of mitochondrial chaperone HSP60 impairs stem cells, but surviving cells promote niche proliferation via WNT factors.

More Related Videos

Intestinal Epithelial Regeneration in Response to Ionizing Irradiation
09:10

Intestinal Epithelial Regeneration in Response to Ionizing Irradiation

Published on: July 27, 2022

2.7K
Author Spotlight: Studying the Epithelial Effects of Intestinal Inflammation In Vitro on Established Murine Colonoids
06:31

Author Spotlight: Studying the Epithelial Effects of Intestinal Inflammation In Vitro on Established Murine Colonoids

Published on: June 2, 2023

1.6K

Related Experiment Videos

Last Updated: Mar 13, 2026

Real Time Analysis of Metabolic Profile in Ex Vivo Mouse Intestinal Crypt Organoid Cultures
08:53

Real Time Analysis of Metabolic Profile in Ex Vivo Mouse Intestinal Crypt Organoid Cultures

Published on: November 3, 2014

16.7K
Intestinal Epithelial Regeneration in Response to Ionizing Irradiation
09:10

Intestinal Epithelial Regeneration in Response to Ionizing Irradiation

Published on: July 27, 2022

2.7K
Author Spotlight: Studying the Epithelial Effects of Intestinal Inflammation In Vitro on Established Murine Colonoids
06:31

Author Spotlight: Studying the Epithelial Effects of Intestinal Inflammation In Vitro on Established Murine Colonoids

Published on: June 2, 2023

1.6K

Area of Science:

  • Cell Biology
  • Gastroenterology
  • Mitochondrial Biology

Background:

  • Intestinal epithelial stemness is critical for gut homeostasis.
  • Dysfunctional epithelial cells are linked to gastrointestinal diseases.
  • Mitochondrial function's role in intestinal stemness is under investigation.

Purpose of the Study:

  • To investigate the role of mitochondrial function in maintaining intestinal stemness.
  • To elucidate the impact of HSP60 loss on intestinal epithelial cells (IECs) and stemness.

Main Methods:

  • Utilized IEC-specific mouse models with altered HSP60 levels.
  • Analyzed mitochondrial function, stemness markers, and cell proliferation.
  • Assessed the mitochondrial unfolded protein response (MT-UPR) and WNT signaling factors.

Main Results:

  • HSP60 deficiency in IECs triggered MT-UPR and mitochondrial dysfunction.
  • HSP60-deficient crypts showed reduced stemness and proliferation.
  • Loss of HSP60 led to WNT10A and RSPO1 release, promoting compensatory stem cell proliferation.

Conclusions:

  • Mitochondrial function, specifically HSP60, is essential for intestinal stemness.
  • Impaired IECs release paracrine factors that influence stem cell niche behavior.
  • HSP60-dependent mitochondrial regulation impacts gastrointestinal tissue homeostasis.