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Updated: Mar 13, 2026

Generation of a Mouse Spontaneous Autoimmune Thyroiditis Model
Published on: March 17, 2023
Eliciting an antibody response against a recombinant TSH containing fusion protein
Maysam Mard-Soltani1, Mohamad Javad Rasaee2, AbdolKarim Sheikhi3
1a Department of Clinical Biochemistry , Tarbiat Modares University , Tehran , Iran.
Abstract:
Designing novel antigens to rise specific antibodies for Thyroid Stimulating Hormone (TSH) detection is of great significance. A novel fusion protein consisting of the C termini sequence of TSH beta subunit and a fusion sequence was designed and produced for rabbit immunization. Thereafter, the produced antibodies were purified and characterized for TSH detection. Our results indicate that the produced antibody is capable of sensitive and specific detection of TSH with low cross reactivity. This study underscores the applicability of designed fusion protein for specific and sensitive polyclonal antibody production and the importance of selecting an amenable region of the TSH for immunization.
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