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Updated: Mar 13, 2026

Isolation and Characterization Of Chimeric Human Fc-expressing Proteins Using Protein A Membrane Adsorbers And A Streamlined Workflow
Published on: January 8, 2014
Isolating Fc-Tagged SEMA4D Recombinant Protein from 293FT Cells
Joanne Soong1,2, Glynis A Scott3,4
1Department of Dermatology, University of Rochester School of Medicine, 601 Elmwood Ave Box 697, Rochester, NY, 14618, USA.
Abstract:
Recombinant proteins are widely used in biomedical sciences, and in pharmaceutical research. Through genetic recombination, specific DNA sequences are engineered and inserted into a biological host. Once inside the host, the DNA is transcribed and translated into the target protein and is ready to be purified. Here, we describe the transfection, purification, and visualization of Fc-tagged SEMA4D (semaphorin 4D) recombinant protein.
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