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Using Rotary Shadow Electron Microscopy to Characterize Semaphorin-Mediated Growth Cone Collapse
1Department of Neuroscience, Washington University School of Medicine, Box 8108, 660 S Euclid, Saint Louis, MO, 63110, USA. bridgmap@wustl.edu.
Methods in Molecular Biology (Clifton, N.J.)
|October 28, 2016
Abstract:
Rotary shadow electron microscopy (EM) of growth cone cytoskeletons provides a high-resolution method for detecting both global and macromolecular changes in cytoskeletal organization or structure. This approach can be used to study responses to repulsive guidance factors such as semaphorin 3A. Here I describe the procedures used to prepare cultured neurons for rotary-shadow EM, allowing detailed comparisons of cytoskeletal structure.
