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Deletions in a recombinant retrovirus genome associated with its expression in embryonal carcinoma cells
1Jackson Laboratory, Bar Harbor, Maine 04609.
Journal of Virology
|October 1, 1989
Abstract:
We analyzed embryonal carcinoma cell lines infected with a recombinant Moloney murine leukemia virus. Lines that carried but did not express the neo gene retained a provirus of LTR-gag-pol-neo-LTR, where LTR is a long terminal repeat, whereas all G418-resistant lines deleted regions that included the primer binding site and the splicing donor site. This suggested the presence of multiple inhibitory elements.