Bromelain: from production to commercialisation.

Area of Science:

• Biochemistry
• Plant Science
• Biotechnology

Background:

• Bromelain is a proteolytic enzyme mixture extracted from pineapple plants (Ananas comosus).
• It is found in various parts of the pineapple plant, including stem, fruit, leaves, and peel.
• Increasing global demand necessitates efficient and cost-effective bromelain production strategies.

Purpose of the Study:

• To review modern purification techniques for bromelain production.
• To discuss the role of recombinant DNA technology in producing ultrapure bromelain.
• To compile data on the commercialization of bromelain in clinical, pharmaceutical, and industrial sectors.

Main Methods:

• Conventional methods: centrifugation, ultrafiltration, lyophilization.
• Modern techniques: gel filtration, ion exchange chromatography, affinity chromatography, aqueous two-phase extraction, reverse micelle chromatography.
• Recombinant DNA technology for large-scale ultrapure bromelain production.

Main Results:

• Advanced purification techniques have significantly increased industrial bromelain production.
• Recombinant DNA technology offers a viable alternative for producing high-purity bromelain.
• The review provides an updated overview of bromelain commercialization.

Conclusions:

• Modern purification and biotechnological approaches are crucial for meeting the growing demand for bromelain.
• These advancements enhance bromelain purity, reduce production costs, and streamline processes.
• Bromelain's commercial applications continue to expand across various industries.