[Effect of lipids on thrombocyte aggregation in patients with ischemic heart disease]

Platelet lipid composition was studied in healthy subjects and patients with coronary heart disease (CHD) and effects of various phospholipids (PL) were examined on aggregatory parameters. The patients with CHD was found to show higher levels of lysophosphatidylcholine (LPC), phosphatidic acid (PA), cholesterol (Chol), increased Chol/PL of saturated fatty acids, elevated malonic dialdehyde and lower phosphatidylinositol (PI) concentrations. Addition of liposomes from various PL classes to platelet-rich plasma indicated as follows. Phosphatidylserine (PS) liposomes caused a decrease in the platelet aggregation both in healthy subjects and CHD patients. Phosphatidylcholine- (PC), phosphatidylethanolamine- (PE), PI-, and LPC-liposomes increased the platelet aggregation in healthy subjects. Incubation of PE- and PI-liposomes with platelet-rich plasma from CHD patients resulted in lower platelet aggregation, whereas that of PC and LPC liposomes led to its increase. In CHD patients, the changes in the platelet lipid composition were likely to occur due to stimulation of lipid peroxidation and activation of platelet phospholipases, and diminished platelet aggregation caused by addition of PI- and PE-liposomes was likely to be associated with reconstruction of lipid composition and reduction of substrates of the phosphoinositide cycle.