Root coverage using porcine collagen matrix with fibroblast growth factor-2: a pilot study in dogs

The aim of this study was to evaluate the effect of fibroblast growth factor-2 (FGF-2) in combination with porcine collagen matrix (CM) for coverage of gingival recession defects in dogs.

In five male mongrel dogs, labial gingival recession defects were surgically created in the lower-third incisors bilaterally. The defects were randomly assigned to the CM/FGF-2 group (experimental) or the CM-only group (control). Standardized clinical photographs and silicone impressions were taken at 4 and 16 weeks of healing to calculate the remaining recession area. The dogs were euthanized after 16 weeks for histometric analysis.

At 4 weeks, the recession area of the CM/FGF-2 group was significantly smaller than that of the CM-only group (2.55 ± 0.66 and 4.92 ± 1.05 mm² , respectively, p = 0.023), However, no significant difference was observed at 16 weeks. Histometrically, the amount of newly formed cementum was larger in the CM/FGF-2 group than in the CM-only group at 16 weeks (1.55 ± 0.44 and 0.88 ± 0.51 mm, respectively, p = 0.024).

The combination of CM/FGF-2 may enhance the early wound healing and may promote cementum formation to a higher extent compared to the use of CM alone.