Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Improving Translational Accuracy

Improving Translational Accuracy

Base complementarity between the three base pairs of mRNA codon and the tRNA anticodon is not a failsafe mechanism. Inaccuracies can range from a single mismatch to no correct base pairing at all. The free energy difference between the correct and nearly correct base pairs can be as small as 3 kcal/ mol. With complementarity being the only proofreading step, the estimated error frequency would be one wrong amino acid in every 100 amino acids incorporated. However, error frequencies observed in...

Uncertainty: Overview

Uncertainty: Overview

In analytical chemistry, we often perform repetitive measurements to detect and minimize inaccuracies caused by both determinate and indeterminate errors. Despite the cares we take, the presence of random errors means that repeated measurements almost never have exactly the same magnitude. The collective difference between these measurements - observed values - and the estimated or expected value is called uncertainty. Uncertainty is conventionally written after the estimated or expected value.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

A continuous-discrete model of cell contraction incorporating actin and intermediate filaments.

iScience·2026

Same author

CLEC14A correlates with neutrophil infiltration in hepatocellular carcinoma and mediates neutrophil recruitment across liver endothelial cells.

The Journal of pathology·2026

Same author

Identifying and mapping key relationships and communication pathways influencing farmers' antibiotic use in production animals: a scoping review.

One health outlook·2026

Same author

Highlighting the need for more holistic, participatory design of assistive technologies in displacement settings.

Frontiers in public health·2026

Same author

Migration corridors in Africa and access to health services: Current challenges and a path forward for research and practice.

Journal of public health in Africa·2026

Same author

Microplastics as active modulators of <i>Escherichia coli</i> biofilm characteristics and their implications on the development of antimicrobial resistance.

Biofilm·2026

Search research articles

Related Experiment Video

Updated: Mar 13, 2026

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

Published on: February 28, 2015

Controlling uncertainty in aptamer selection.

Fabian Spill¹, Zohar B Weinstein², Atena Irani Shemirani³

¹Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139; Department of Biomedical Engineering, Boston University, Boston MA 02215.

Proceedings of the National Academy of Sciences of the United States of America

|October 30, 2016

Summary

This summary is machine-generated.

Discovering high-affinity aptamers using Systematic Evolution of Ligands by EXponential Enrichment (SELEX) is challenging. Our model reveals that even rare aptamers can impact SELEX dynamics, highlighting the role of chance and environmental factors.

Keywords:

SELEX aptamer evolutionary dynamics hybrid model stochastic process

More Related Videos

Primer-Free Aptamer Selection Using A Random DNA Library

Primer-Free Aptamer Selection Using A Random DNA Library

Published on: July 26, 2010

A Flow Cytometry-Based Cell Surface Protein Binding Assay for Assessing Selectivity and Specificity of an Anticancer Aptamer

A Flow Cytometry-Based Cell Surface Protein Binding Assay for Assessing Selectivity and Specificity of an Anticancer Aptamer

Published on: September 13, 2022

Related Experiment Videos

Last Updated: Mar 13, 2026

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

Published on: February 28, 2015

Primer-Free Aptamer Selection Using A Random DNA Library

Primer-Free Aptamer Selection Using A Random DNA Library

Published on: July 26, 2010

A Flow Cytometry-Based Cell Surface Protein Binding Assay for Assessing Selectivity and Specificity of an Anticancer Aptamer

A Flow Cytometry-Based Cell Surface Protein Binding Assay for Assessing Selectivity and Specificity of an Anticancer Aptamer

Published on: September 13, 2022

Area of Science:

Biotechnology
Molecular Biology
Bioinformatics

Background:

Aptamer discovery via Systematic Evolution of Ligands by EXponential Enrichment (SELEX) is crucial for molecular recognition.
SELEX relies on evolutionary selection from diverse random pools, but outcomes can be variable and unpredictable.
Understanding the factors influencing SELEX efficiency is vital for optimizing aptamer selection.

Purpose of the Study:

To develop a stochastic hybrid model to simulate aptamer evolutionary selection.
To investigate the impact of uncertainties and environmental parameters on SELEX outcomes.
To identify strategies for improving the efficiency and speed of aptamer discovery.

Main Methods:

Development of a stochastic hybrid model for aptamer evolutionary dynamics.
Monte Carlo simulations to explore SELEX under varying conditions.
Analysis of population dynamics influenced by high-affinity aptamer presence.

Main Results:

Even single high-affinity aptamers can significantly influence population dynamics in SELEX.
Aptamer survival is highly dependent on stochastic events and chance.
Target concentration critically affects SELEX efficiency, with strategies identified to mitigate uncertainties.

Conclusions:

The stochastic model provides insights into the complex dynamics of aptamer SELEX.
Controlling environmental parameters like target concentration can enhance selection efficiency.
This work offers strategies to accelerate and improve the resource-intensive aptamer discovery process.