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Updated: Mar 12, 2026
Quantifying the Binding Interactions Between CuII and Peptide Residues in the Presence and Absence of Chromophores
Published on: April 5, 2022
A lipophilic copper(ii) complex as an optical probe for intracellular detection of NO
Neil Wilson1, Lok Hang Mak2, Agostino Cilibrizzi1
1Department of Chemistry, Imperial College London, London SW7 2AZ, UK and Institute of Chemical Biology, Imperial College London, London SW7 2AZ, UK. r.woscholski@imperial.ac.uk r.vilar@imperial.ac.uk.
Abstract:
A new chemical sensor for cellular imaging of NO is presented. This cell-permeable probe is based on a complex where copper(ii) is coordinated to a tridentate ligand substituted with a fluorophore (NBD) and an octyl group. The fluorescence response of this complex towards a range of reactive species (namely NO, NO2-, NO3-, H2O2, ClO-, O2- and ONOO-) has been studied in vitro showing that the probe is highly selective for NO. The probe is readily taken up by cells and is able to image the cellular concentrations of NO.
