Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Parental conflict driven regulation of endosperm cellularization by a family of Auxin Response Factors.

Nature plants·2024

Same author

Modification of spin-ice physics in <math><mrow><msub><mrow><mtext>Ho</mtext></mrow><mn>2</mn></msub><msub><mrow><mtext>Ti</mtext></mrow><mn>2</mn></msub><msub><mtext>O</mtext><mn>7</mn></msub></mrow></math> thin films.

Physical review materials·2024

Same author

[Influence of paraspinal muscle degeneration and postoperative Roussouly classification restoration on mechanical complications in female patients with degenerative scoliosis after surgery].

Zhonghua yi xue za zhi·2024

Same author

[Molecular epidemiological characteristics of newly diagnosed HIV-1 cases in Fujian Province in 2020].

Zhongguo xue xi chong bing fang zhi za zhi = Chinese journal of schistosomiasis control·2024

Same author

[Characterization of a 3-dimensional tuberculosis spheroid model constructed using human monocytic THP-1 cells and <i>Bacillus Calmette-Guerin</i>].

Nan fang yi ke da xue xue bao = Journal of Southern Medical University·2024

Same author

[Clinical study of the Cobb+1 to Cobb fusion strategy for Lenke 5C adolescent idiopathic scoliosis patients with the lower lumbar apex].

Zhonghua yi xue za zhi·2024

Search research articles

Related Experiment Video

Updated: Mar 12, 2026

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Published on: October 28, 2018

Single-Molecule Imaging With One Color Fluorescence.

Y Qiu¹, S Myong¹

¹Johns Hopkins University, Baltimore, MD, United States.

Methods in Enzymology

|October 30, 2016

Summary

This summary is machine-generated.

Single-molecule protein-induced fluorescence enhancement (PIFE) offers a precise, single-dye method for observing biomolecular interactions. This technique enhances fluorescence upon protein binding, providing a valuable alternative to Förster resonance energy transfer (FRET) for studying protein-nucleic acid dynamics.

Keywords:

Förster resonance energy transfer One-color imaging Protein–nucleic acid interactions Single-molecule protein-induced fluorescence enhancement

More Related Videos

Compact Quantum Dots for Single-molecule Imaging

Compact Quantum Dots for Single-molecule Imaging

Published on: October 9, 2012

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

Related Experiment Videos

Last Updated: Mar 12, 2026

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Published on: October 28, 2018

Compact Quantum Dots for Single-molecule Imaging

Compact Quantum Dots for Single-molecule Imaging

Published on: October 9, 2012

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

Area of Science:

Biophysics
Molecular Biology
Biochemistry

Background:

Single-molecule fluorescence imaging allows real-time observation of molecular interactions.
Förster resonance energy transfer (FRET) is a common technique but requires both donor and acceptor dyes.
Protein-nucleic acid interactions are fundamental to many biological processes.

Purpose of the Study:

To provide a detailed procedure for the single-molecule protein-induced fluorescence enhancement (smPIFE) method.
To highlight smPIFE as a powerful tool for studying biomolecular interactions.
To discuss the applications of smPIFE in protein-nucleic acid interaction studies.

Main Methods:

Utilizes a single fluorescent dye, unlike FRET.
Measures the increase in fluorescence intensity upon protein binding near the dye.
Demonstrates distance sensitivity within a 0-4nm range.

Main Results:

smPIFE is a sensitive technique for detecting protein binding events.
The method provides nanometer precision in observing molecular dynamics.
It serves as a complementary or alternative approach to FRET.

Conclusions:

smPIFE is a versatile and effective single-molecule fluorescence method.
The technique offers advantages in simplicity and applicability for studying protein-nucleic acid interactions.
Further exploration of smPIFE applications is warranted.