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Related Experiment Videos

Bleach and LISS: a potential hazard.

T M Annesley, W J Judd

    Medical Laboratory Sciences
    |January 1, 1989
    PubMed
    Summary

    Copper alloy corrosion occurred due to an exothermic reaction between copper, glycine, and sodium hypochlorite (bleach). This highlights risks when using bleach with glycine-containing materials near base metal laboratory equipment.

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    Area of Science:

    • Materials Science
    • Biochemistry
    • Laboratory Safety

    Background:

    • Laboratory apparatus, specifically copper alloy components, can be susceptible to chemical degradation.
    • Low-ionic-strength saline (LISS) is used in blood sample preparation, and glycine is its primary constituent.
    • Sodium hypochlorite (bleach) is a common disinfectant in laboratory settings.

    Purpose of the Study:

    • To investigate the cause of corrosion observed in a copper alloy inlet port of a vacuum aspirator.
    • To identify the chemical reaction responsible for the degradation of laboratory equipment.
    • To assess the safety implications of using sodium hypochlorite with glycine-containing solutions.

    Main Methods:

    • Observation of equipment failure (corrosion hole).
    • Analysis of the chemical composition of the aspirator's fluid (LISS) and cleaning agent (sodium hypochlorite).
    • Identification of the reaction between copper, glycine, and sodium hypochlorite.

    Main Results:

    • An exothermic reaction was identified between copper, glycine, and sodium hypochlorite.
    • Corrosion of the copper alloy inlet port occurred, forming a hole.
    • The reaction was observed in an apparatus used for aspirating LISS-wash supernatants.

    Conclusions:

    • Caution is advised when using sodium hypochlorite to decontaminate glycine-containing waste or spills.
    • Direct contact between sodium hypochlorite, glycine, and base metal laboratory apparatus, particularly copper, should be avoided.
    • The findings underscore the importance of material compatibility in laboratory procedures and cleaning protocols.

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