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Published on: July 28, 2012
Ad-shWnt2b Vector Therapy Demonstrates Antitumor Activity in Orthotopic Intrapleural Models as Monitored with the In
Masashi Kobayashi1, Cheng-Long Huang2, Makoto Sonobe2
1Department of Thoracic Surgery, Tokyo Medical and Dental University, Tokyo, Japan mkobthsr@tmd.ac.jp.
Aim:
We investigated whether Wnt2B-inhibiting therapy has effective antitumor activity against Wnt2B-overexpressing cells in an orthotopic intrapleural model by monitoring with the in vitro imaging system (IVIS).
Materials And Methods:
Mice for the orthotopic intrapleural model were randomized into 3 groups (control, Ad-shWnt2B and Ad-scramble; 8 mice per group). The respective vector was injected into the intrapleural cavity of mice in the Ad-shWnt2B and Ad-scramble groups. After 6 weeks of vector treatment, all mice were monitored with the IVIS. Additionally, their body weight was measured until all mice died from the tumor or were sacrificed.
Results:
A549-Luc-positive cells showed cytotoxicity following exposure to the Ad-shWnt2B vector. The percentage of viable cells was significantly lower in A549-Luc cells treated with Ad-shWnt2B than with Ad-scramble (p<0.01 versus control or Ad-scramble, respectively).
Insights
Wnt2B-inhibiting therapy demonstrated significant antitumor effects in an orthotopic intrapleural model. Treatment with Ad-shWnt2B effectively reduced tumor cell viability in Wnt2B-overexpressing cells.
Area of Science:
- Oncology
- Gene Therapy
Background:
- Wnt2B overexpression is implicated in certain cancers.
- Targeting Wnt2B signaling is a potential therapeutic strategy.
Purpose of the Study:
- To evaluate the antitumor efficacy of Wnt2B-inhibiting therapy in an orthotopic intrapleural model.
- To assess the impact of Ad-shWnt2B on Wnt2B-overexpressing tumor cells.
Main Methods:
- An orthotopic intrapleural mouse model was established using A549-Luc cells.
- Mice were randomized into control, Ad-shWnt2B, and Ad-scramble groups.
- In vivo imaging system (IVIS) and body weight measurements were used for monitoring.
Main Results:
- Ad-shWnt2B treatment resulted in significant cytotoxicity in A549-Luc cells.
- The percentage of viable cells was significantly lower in the Ad-shWnt2B group compared to control and Ad-scramble groups (p<0.01).
Conclusions:
- Wnt2B-inhibiting therapy shows promising antitumor activity against Wnt2B-overexpressing intrapleural tumors.
- Ad-shWnt2B is an effective agent for reducing tumor cell viability in this model.
