Related Experiment Video
Updated: Mar 12, 2026

12:08
Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies
Published on: August 20, 2021
5.9K
Draft Genome Sequence of Ureolytic Environmental Isolate Staphylococcus sp. NA309
Jonathan R Gaiero1, Tom Hsiang1, Rob W Nicol1
1School of Environmental Sciences, University of Guelph, Guelph, Ontario, Canada.
Genome Announcements
|November 1, 2016
Summary
We sequenced the genome of Staphylococcus sp. NA309 from poultry litter. This bacterium generates ammonia due to its ureolytic activity, impacting litter residue.
Area of Science:
- Microbiology
- Genomics
- Environmental Science
Background:
- Poultry litter is a complex environment with significant microbial activity.
- Ammonia generation in poultry litter impacts air quality and nutrient cycling.
Purpose of the Study:
- To perform a draft genome sequencing of Staphylococcus sp. NA309.
- To characterize the ureolytic activity of this dominant poultry litter isolate.
Main Methods:
- Draft genome sequencing of Staphylococcus sp. NA309.
- Isolation and identification of dominant cultivable aerobic bacteria from poultry litter.
- Assay for ureolytic activity.
Main Results:
- A 2.7 Mb draft genome sequence for Staphylococcus sp. NA309 was obtained.
- Staphylococcus sp. NA309 was identified as a dominant aerobic bacterium in the poultry litter.
- The isolate demonstrated significant ureolytic activity, indicating its role in ammonia production.
Conclusions:
- The genome sequence provides a basis for understanding the metabolic capabilities of Staphylococcus sp. NA309.
- Staphylococcus sp. NA309 is a key contributor to ammonia generation in poultry litter ecosystems.
- Understanding these bacteria is crucial for managing poultry litter quality and environmental impact.

