Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

MRI-Only Simulation for Prostate MRI-Guided SBRT.

Practical radiation oncology·2026
Same author

Durability of Response to Icotrokinra in Adults With Moderate-to-Severe Plaque Psoriasis: One-Year Results From the Phase 3, Placebo- and Active Comparator-Controlled ICONIC-ADVANCE 1 & ICONIC-ADVANCE 2 Trials.

The British journal of dermatology·2026
Same author

RNU4ATAC-opathy: Clinical, molecular and transcriptomic insights from a large cohort.

Genetics in medicine : official journal of the American College of Medical Genetics·2026
Same author

Linking primary care data from clinical practice research datalink to secondary care and other health-related patient data: update and implications.

International journal of population data science·2026
Same author

Investigation of Methacryloyl-Modified Gelatin-Based Hydrogels for Inkjet-Printed Biosensors and Their Adherence to Polyethylene Terephthalate and Oriented Polypropylene Substrates.

Engineering in life sciences·2026
Same author

Cetrorelix suppresses the dominant follicle and synchronizes follicular waves and ovulation in cattle†.

Biology of reproduction·2025

Related Experiment Video

Updated: Mar 12, 2026

Stress-induced Antibiotic Susceptibility Testing on a Chip
12:41

Stress-induced Antibiotic Susceptibility Testing on a Chip

Published on: January 8, 2014

7.0K

Microfluidic advances in phenotypic antibiotic susceptibility testing.

Jennifer Campbell1, Christine McBeth1, Maxim Kalashnikov1

  • 1Fraunhofer USA - Center for Manufacturing Innovation, Brookline, MA, 02446, USA.

Biomedical Microdevices
|November 1, 2016
PubMed
Summary
This summary is machine-generated.

Microfluidic devices offer rapid antibiotic susceptibility testing (AST) to combat rising microbial resistance. These innovative platforms accelerate diagnosis, enabling quicker treatment decisions and improving patient outcomes.

Keywords:
Antibiotic susceptibility testingBacterial growthMicrofluidic methodologiesPoint-of-careRapid phenotypic screening

More Related Videos

Rapid Antimicrobial Susceptibility Testing by Stimulated Raman Scattering Imaging of Deuterium Incorporation in a Single Bacterium
12:08

Rapid Antimicrobial Susceptibility Testing by Stimulated Raman Scattering Imaging of Deuterium Incorporation in a Single Bacterium

Published on: February 14, 2022

3.5K
Design and Use of a Low Cost, Automated Morbidostat for Adaptive Evolution of Bacteria Under Antibiotic Drug Selection
10:50

Design and Use of a Low Cost, Automated Morbidostat for Adaptive Evolution of Bacteria Under Antibiotic Drug Selection

Published on: September 27, 2016

10.2K

Related Experiment Videos

Last Updated: Mar 12, 2026

Stress-induced Antibiotic Susceptibility Testing on a Chip
12:41

Stress-induced Antibiotic Susceptibility Testing on a Chip

Published on: January 8, 2014

7.0K
Rapid Antimicrobial Susceptibility Testing by Stimulated Raman Scattering Imaging of Deuterium Incorporation in a Single Bacterium
12:08

Rapid Antimicrobial Susceptibility Testing by Stimulated Raman Scattering Imaging of Deuterium Incorporation in a Single Bacterium

Published on: February 14, 2022

3.5K
Design and Use of a Low Cost, Automated Morbidostat for Adaptive Evolution of Bacteria Under Antibiotic Drug Selection
10:50

Design and Use of a Low Cost, Automated Morbidostat for Adaptive Evolution of Bacteria Under Antibiotic Drug Selection

Published on: September 27, 2016

10.2K

Area of Science:

  • Biotechnology and Biomedical Engineering
  • Microfluidics
  • Antimicrobial Resistance

Background:

  • Extensive antibiotic use and misuse have driven significant microbial resistance.
  • Current antibiotic susceptibility testing (AST) methods require 48-72 hours, delaying critical treatment decisions.
  • Broad-spectrum antibiotic prescription is a major driver of resistance, exacerbated by slow diagnostic turnaround times.

Purpose of the Study:

  • To review recent advances in microfluidic-based phenotypic antibiotic susceptibility testing (AST).
  • To highlight the potential of microfluidics to overcome limitations of traditional AST methods.
  • To identify key challenges and future directions in microfluidic AST development.

Main Methods:

  • Review of microfluidic platforms for phenotypic antibiotic susceptibility testing (AST) published in the last two years.
  • Categorization of methods based on chip functionality, including microscopy, gradient generators, and antibody-based capture.
  • Inclusion of both growth-dependent and growth-independent AST approaches.

Main Results:

  • Microfluidic devices offer advantages such as faster assays, increased multiplexing, smaller sample volumes, and potential for point-of-care use.
  • Advances span simple microscopy to sophisticated gradient generators and antibody-based capture systems.
  • Both growth-monitoring and non-growth-based microfluidic AST methods have been developed.

Conclusions:

  • Microfluidic phenotypic AST shows significant promise for rapid and efficient antibiotic susceptibility determination.
  • Key hurdles remain in sample preparation and cost-effective detection technologies for widespread adoption.
  • Disruptive microfluidic approaches are crucial to address the antibiotic resistance crisis.