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A Sister Chromatid Cohesion Assay Using Xenopus Egg Extracts

Keishi Shintomi1, Tatsuya Hirano2

  • 1Chromosome Dynamics Laboratory, RIKEN, 2-1 Hirosawa, Wako, Saitama, 351-0198, Japan.

Methods in Molecular Biology (Clifton, N.J.)
|November 1, 2016
PubMed
Summary

Researchers used Xenopus laevis egg extracts to study cell cycle progression and chromosome duplication. They developed methods to analyze sister chromatid cohesion, a key event in cell division.

Keywords:
Cell-free assayCohesinCondensinsImmunodepletionImmunofluorescenceSister chromatid cohesionXenopus egg extracts

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