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Updated: Mar 12, 2026
11:25
A G-quadruplex DNA-affinity Approach for Purification of Enzymatically Active G4 Resolvase1
Published on: March 18, 2017
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The novel G-quadruplex-containing long non-coding RNA GSEC antagonizes DHX36 and modulates colon cancer cell
K Matsumura1, Y Kawasaki1, M Miyamoto1
1Laboratory of Molecular and Genetic Information, Institute of Molecular and Cellular Bioscience, The University of Tokyo, Tokyo, Japan.
Oncogene
|November 1, 2016
Summary
A novel long non-coding RNA, GSEC, is upregulated in colorectal cancer and promotes cancer cell migration by inhibiting the DHX36 RNA helicase. This finding offers new insights into colorectal cancer progression.
Area of Science:
- Molecular Biology
- Oncology
Background:
- Long non-coding RNAs (lncRNAs) are increasingly recognized for their roles in cancer, but their specific functions in colorectal cancer are not fully understood.
- Dysregulation of lncRNAs is a common feature in various human malignancies, highlighting their potential as biomarkers and therapeutic targets.
Purpose of the Study:
- To identify and characterize novel lncRNAs involved in colorectal cancer pathogenesis.
- To elucidate the molecular mechanisms by which identified lncRNAs regulate cancer cell behavior, specifically focusing on cell motility.
Main Methods:
- Gene expression profiling of colon cancer tissues to identify differentially expressed lncRNAs.
- Functional assays, including knockdown experiments, to assess the impact of GSEC on colon cancer cell motility.
- Biochemical assays to investigate the interaction between GSEC and DHX36 RNA helicase and its effect on G-quadruplex unwinding activity.
Main Results:
- Identification of a novel lncRNA, GSEC (FLJ39051), upregulated in colorectal cancer.
- Knockdown of GSEC significantly reduces colon cancer cell migration.
- GSEC directly binds to DHX36 RNA helicase, inhibiting its G-quadruplex unwinding activity, and DHX36 knockdown rescues GSEC knockdown-induced reduction in cell migration.
Conclusions:
- GSEC promotes colon cancer cell migration by inhibiting DHX36 RNA helicase activity through its G-quadruplex structure.
- GSEC represents a potential therapeutic target for colorectal cancer treatment.
- Understanding the lncRNA-protein interactions in cancer provides new avenues for therapeutic intervention.
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