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Updated: Mar 12, 2026
Monitoring Kinase and Phosphatase Activities Through the Cell Cycle by Ratiometric FRET
Published on: January 27, 2012
Fluorescent indicators for simultaneous reporting of all four cell cycle phases
Bryce T Bajar1,2,3, Amy J Lam1,2, Ryan K Badiee4
1Department of Bioengineering, Stanford University, Stanford, California, USA.
Abstract:
A robust method for simultaneous visualization of all four cell cycle phases in living cells is highly desirable. We developed an intensiometric reporter of the transition from S to G2 phase and engineered a far-red fluorescent protein, mMaroon1, to visualize chromatin condensation in mitosis. We combined these new reporters with the previously described Fucci system to create Fucci4, a set of four orthogonal fluorescent indicators that together resolve all cell cycle phases.
