Separation of Biologically Active Compounds by Membrane Operations.
1Department of Civil and Environmental Engineering, National University of Singapore, 1 Engineering Drive 2, Singapore.
Current Pharmaceutical Design
|November 2, 2016
Summary
Membrane separation technology offers an energy-efficient and gentle method for isolating valuable bioactive compounds from natural sources. This approach overcomes limitations of conventional methods, enabling effective concentration and purification.
Area of Science:
- Biotechnology
- Chemical Engineering
- Natural Product Chemistry
Background:
- Bioactive compounds from natural sources are gaining attention for their diverse functionalities.
- Low concentrations in natural mixtures necessitate efficient separation technologies for practical use.
- Conventional separation methods can be energy-intensive and may degrade sensitive compounds.
Purpose of the Study:
- To review membrane separation processes for isolating bioactive compounds.
- To highlight the advantages of membrane technology over traditional methods.
- To discuss current and future trends in membrane-based bioactive compound separation.
Main Methods:
- Comprehensive survey of established membrane separation operations.
- Review of pressure-driven processes: microfiltration (MF), ultrafiltration (UF), and nanofiltration (NF).
- Exploration of advanced techniques like hybrid electrodialysis-UF and affinity membrane technology.
Main Results:
- Pressure-driven membrane processes (MF, UF, NF) are effective individually or in arrays for separating compounds by size.
- Hybrid and affinity membrane processes offer solutions for separating molecules with similar sizes but different properties.
- Membrane fouling remains a challenge, but surface modification and multifunctional membranes show promise.
Conclusions:
- Membrane technology is a promising, energy-efficient approach for concentrating and separating bioactive compounds.
- Advanced membrane designs and surface modifications can enhance separation efficiency and mitigate fouling.
- Further research into optimizing membranes and operational parameters is crucial for diverse bioactive compound applications.
More Related Videos
06:14Separation and Fractionation of Cell Wall and Cell Membrane Proteins from Mycobacterium tuberculosis for Downstream Protein Analysis
Published on: September 26, 2025
798
10:24Separation of the Cell Envelope for Gram-negative Bacteria into Inner and Outer Membrane Fractions with Technical Adjustments for Acinetobacter baumannii
Published on: April 10, 2020
14.5K
Related Concept Videos
01:20Overview Of Cell Separation And Isolation
8.0K
Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.
8.0K
01:24Cellular Membranes and Drug Transport
2.0K
Drugs must traverse multiple biological barriers, such as multi-layered skin, single-layered intestinal epithelium, and the plasma membrane, to reach their target sites within the body. The plasma membrane, a highly structured composite of phospholipids, carbohydrates, and proteins, is the cell's protective boundary, facilitating selective substance exchange.
Phospholipids arrange themselves into a bilayer, with hydrophilic heads oriented outward and hydrophobic tails facing inward.
Phospholipids arrange themselves into a bilayer, with hydrophilic heads oriented outward and hydrophobic tails facing inward.
2.0K
01:20Factors Affecting Dissolution: Drug Permeability, Stability and Stereochemistry
643
Orally administered drugs primarily enter the systemic circulation via passive diffusion through the intestinal membranes. The drug's absorption is influenced by drug stability in the gastrointestinal GI tract, membrane permeability, the surface area available for absorption, luminal drug concentration, and residence time in the lumen. Drug permeability can be enhanced by adjusting the lipophilicity, polarity, or molecular size of the drug, promoting its passive transport across intestinal...
643
01:15Dialysis
2.1K
Dialysis is a diffusion-based purification process that separates analyte molecules from a complex matrix. This is accomplished by allowing molecules in the solution to pass through a semipermeable membrane into a liquid on the other side. The membrane is usually made of cellulose acetate or cellulose nitrate, and the second liquid must be miscible with the solution. Ions (e.g., chloride or sodium) or organic molecules (e.g., glucose) can pass through the membrane pores, which generally have...
2.1K
01:32Subcellular Fractionation
9.4K
The homogenate obtained after cell lysis contains various membrane-bound organelles that can be further separated into pure fractions by subcellular fractionation. These isolates are used to study specific cellular components, analyze localized protein activity, and are even employed in diagnostics. Fractionation is typically achieved using centrifugation methods, the most common being density-gradient and differential centrifugation.
Differential Centrifugation
Differential centrifugation is...
Differential Centrifugation
Differential centrifugation is...
9.4K
01:23Mechanisms of Drug Absorption: Paracellular, Transcellular, and Vesicular Transport
2.3K
Drugs need to permeate cell membranes to reach their target sites after administration. Orally administered drugs must transcend intestinal epithelial membrane barriers to infiltrate the systemic circulation. Drugs with a molecular weight of less than 500 Daltons diffuse through gaps between neighboring cells, called paracellular pathways.
However, most drugs use the transcellular route, traversing directly through the cell membranes via two mechanisms: passive and active transport. Passive...
However, most drugs use the transcellular route, traversing directly through the cell membranes via two mechanisms: passive and active transport. Passive...
2.3K