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Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
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Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis
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Label-free DNA-based biosensors using structure-selective light-up dyes.

Yahui Guo1, Lijun Xu2, Shanni Hong2

  • 1State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.

The Analyst
|November 2, 2016
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Summary
This summary is machine-generated.

Label-free biosensors (LFBs) utilize structure-selective dyes for DNA detection. This review highlights recent advances in DNA-based LFBs for sensitive and reliable analysis.

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Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Molecular Biology

Background:

  • Label-free biosensors (LFBs) offer cost-effective detection methods.
  • DNA-based LFBs commonly rely on binding-induced structural changes for signal generation.

Purpose of the Study:

  • To review recent studies on DNA-based LFBs utilizing structure-selective nucleic acid dyes.
  • To categorize these biosensors based on dye selectivity for various DNA conformations.

Main Methods:

  • Collection and structuring of recent research on DNA-based LFBs.
  • Categorization based on dye binding affinity to specific DNA structures (ssDNA, dsDNA, triplex, i-motif, G-quadruplex).
  • Inclusion of studies incorporating nucleases, nanomaterials, logic gates, and DNA nanostructures.

Main Results:

  • Demonstrated application of structure-selective dyes in developing sensitive DNA biosensors.
  • Integration of advanced components like nanomaterials and logic systems enhances biosensor functionality.
  • Successful detection across diverse DNA conformations including single-stranded, double-stranded, triplex, i-motif, and G-quadruplex structures.

Conclusions:

  • DNA-based LFBs using structure-selective dyes show significant potential for advanced diagnostics.
  • Future development will focus on ultrahigh sensitivity, intelligent analysis, and portable, rapid assays.
  • Continued innovation is expected to drive the field of label-free biosensing forward.