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Identification of a Plasmid-Mediated Quinolone Resistance Gene in Salmonella Isolates from Texas Dairy Farm

K J Cummings1, L D Rodriguez-Rivera1, K N Norman1

  • 1Texas A&M University, College Station, TX, USA.

Zoonoses and Public Health
|November 2, 2016
PubMed

Insights

Plasmid-mediated quinolone resistance (PMQR) in Salmonella was detected in US dairy farm environments. This finding is crucial for understanding the sources of human Salmonella infections linked to antimicrobial resistance.

Area of Science:

  • Microbiology
  • Antimicrobial Resistance
  • Food Safety

Background:

  • A rise in plasmid-mediated quinolone resistance (PMQR) in human Salmonella isolates in the US necessitates identifying infection sources.
  • Previous work isolated Salmonella from Texas dairy farms, prompting antimicrobial susceptibility testing.

Purpose of the Study:

  • To investigate the presence of PMQR in Salmonella from US dairy farm environments.
  • To identify the genetic basis of quinolone resistance in these isolates.

Main Methods:

  • Antimicrobial susceptibility testing of previously isolated Salmonella from dairy farm environments.
  • Whole-genome sequencing of Salmonella isolates exhibiting quinolone resistance patterns.
  • Identification of resistance genes and plasmid types.

Main Results:

  • Two Salmonella Muenster isolates from a Texas dairy farm showed resistance to nalidixic acid and intermediate resistance to ciprofloxacin.
  • Whole-genome sequencing identified the plasmid-mediated qnrB19 gene and IncR plasmid type in both isolates.
  • This marks the first report of PMQR in Salmonella from US food animals or agricultural settings.

Conclusions:

  • The qnrB19 gene on an IncR plasmid is present in Salmonella from US dairy environments.
  • Dairy farms may serve as a reservoir for PMQR Salmonella, potentially contributing to human infections.
  • This discovery highlights the importance of monitoring agricultural environments for antimicrobial resistance.
Keywords:
Antimicrobial resistanceSalmonella sppdairy cattlefoodborne diseasepublic health

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