Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Regulation of Angiogenesis and Blood Supply01:24

Regulation of Angiogenesis and Blood Supply

3.9K
Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits.  Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
3.9K
Mechanism of Angiogenesis01:10

Mechanism of Angiogenesis

7.4K
Blood vessel formation starts early during embryonic development, around day 7. In the extraembryonic yolk sac, mesodermal precursor cells called hemangioblast proliferate and differentiate into angioblast. Angioblasts express vascular endothelial growth factor receptor 2 or VEGFR2, which binds VEGF-A, a proangiogenic factor, guiding blood vessel formation. VEGF signaling promotes angioblasts to form a blood island in the developing embryo. Angioblasts further differentiate, giving rise to...
7.4K
Overview of the Vascular System01:20

Overview of the Vascular System

3.7K
The vascular system comprises an extensive network of arteries, capillaries, and veins. The vascular system can be broadly divided into the blood and lymphatic systems. Typically, blood vessels can be categorized into three histological regions: tunica intima, tunica media, and tunica adventitia. The tunica intima consists of a single layer of endothelial cells attached to the basal lamina. Underlying the basal lamina is a connective tissue layer and an elastic lamina that gives stability and...
3.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mechanisms of parasite-mediated disruption of brain vessels.

FEBS letters·2025
Same author

Interphase cell morphology defines the mode, symmetry, and outcome of mitosis.

Science (New York, N.Y.)·2025
Same author

A non-genetic model of vascular shunts informs on the cellular mechanisms of formation and resolution of arteriovenous malformations.

Cardiovascular research·2024
Same author

Myonuclear position and blood vessel organization during skeletal muscle postnatal development.

Development (Cambridge, England)·2024
Same author

3DVascNet: An Automated Software for Segmentation and Quantification of Mouse Vascular Networks in 3D.

Arteriosclerosis, thrombosis, and vascular biology·2024
Same author

Cell painting transfer increases screening hit rate.

Biological imaging·2024

Related Experiment Video

Updated: Mar 12, 2026

In Vitro Model of Physiological and Pathological Blood Flow with Application to Investigations of Vascular Cell Remodeling
07:30

In Vitro Model of Physiological and Pathological Blood Flow with Application to Investigations of Vascular Cell Remodeling

Published on: November 3, 2015

10.1K

Endothelial cell dynamics in vascular remodelling.

Pedro Barbacena, Joana R Carvalho, Claudio A Franco

    Clinical Hemorheology and Microcirculation
    |November 2, 2016
    PubMed
    Summary
    This summary is machine-generated.

    Vessel pruning, a key part of vascular remodelling, is driven by endothelial cell reorganization. Non-canonical Wnt signaling controls cell sensitivity to blood flow, stabilizing new vascular networks.

    Keywords:
    Endothelial cellsnon-canonical Wnt signallingshear stressvascular remodelling

    More Related Videos

    Development and Characterization of In Vitro Microvessel Network and Quantitative Measurements of Endothelial [Ca2+]i and Nitric Oxide Production
    09:39

    Development and Characterization of In Vitro Microvessel Network and Quantitative Measurements of Endothelial [Ca2+]i and Nitric Oxide Production

    Published on: May 19, 2016

    9.1K
    Isolation and Culture Expansion of Tumor-specific Endothelial Cells
    10:15

    Isolation and Culture Expansion of Tumor-specific Endothelial Cells

    Published on: October 14, 2015

    11.7K

    Related Experiment Videos

    Last Updated: Mar 12, 2026

    In Vitro Model of Physiological and Pathological Blood Flow with Application to Investigations of Vascular Cell Remodeling
    07:30

    In Vitro Model of Physiological and Pathological Blood Flow with Application to Investigations of Vascular Cell Remodeling

    Published on: November 3, 2015

    10.1K
    Development and Characterization of In Vitro Microvessel Network and Quantitative Measurements of Endothelial [Ca2+]i and Nitric Oxide Production
    09:39

    Development and Characterization of In Vitro Microvessel Network and Quantitative Measurements of Endothelial [Ca2+]i and Nitric Oxide Production

    Published on: May 19, 2016

    9.1K
    Isolation and Culture Expansion of Tumor-specific Endothelial Cells
    10:15

    Isolation and Culture Expansion of Tumor-specific Endothelial Cells

    Published on: October 14, 2015

    11.7K

    Area of Science:

    • Developmental biology
    • Vascular biology
    • Bioengineering

    Background:

    • Vascular network formation involves extensive vessel remodeling.
    • Blood flow is a known driver of vascular remodeling.
    • Vessel pruning is crucial for physiological vascular remodeling, but its mechanisms remain unclear.

    Purpose of the Study:

    • To investigate the cellular and molecular mechanisms regulating developmental vascular remodeling.
    • To understand the interaction between vessel regression and blood flow patterns.
    • To elucidate the role of Wnt signaling in endothelial cell response to shear stress.

    Main Methods:

    • Studied developmental vascular remodeling in mouse and zebrafish models.
    • Developed an in silico method for computing hemodynamic forces in murine retinal vasculature.
    • Utilized network-level analysis and microfluidics to assess endothelial cell behavior.

    Main Results:

    • Identified polarized reorganization of endothelial cells as the core mechanism of vessel regression.
    • Created the first axial polarity map for endothelial cells.
    • Demonstrated that non-canonical Wnt signaling modulates endothelial sensitivity to shear forces.
    • Showed that loss of Wnt5a/11 increases endothelial sensitivity to shear, leading to earlier axial polarization.

    Conclusions:

    • Endothelial cell polarity and reorganization drive vessel regression during vascular development.
    • Non-canonical Wnt signaling stabilizes forming vascular networks by reducing endothelial shear sensitivity.
    • This mechanism prevents vessel closure under low flow conditions in the primitive vascular plexus.