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Related Experiment Videos

Hypervariability of simple sequences as a general source for polymorphic DNA markers.

D Tautz1

  • 1Max-Planck Institut für Entwicklungsbiologie, Tübingen, FRG.

Nucleic Acids Research
|August 25, 1989
PubMed
Summary

Short simple sequence repeats, also known as simple sequence length polymorphisms (SSLP), are highly variable genomic elements. Polymerase chain reaction (PCR) analysis confirms their extensive length polymorphisms, useful for genetic studies.

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Area of Science:

  • Genomics and Molecular Biology
  • Population Genetics

Background:

  • Short simple sequence stretches are highly repetitive elements found in eukaryotic and prokaryotic genomes.
  • These repetitive sequences are hypothesized to arise from slippage events on internally repetitive DNA, predicting length variability.

Purpose of the Study:

  • To investigate the length variability of randomly selected simple sequence loci.
  • To demonstrate the utility of simple sequence length polymorphisms (SSLP) in various genetic applications.

Main Methods:

  • Utilized the polymerase chain reaction (PCR) technique.
  • Analyzed several randomly chosen simple sequence loci with varying nucleotide compositions and from different species.

Main Results:

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  • Demonstrated extensive length polymorphisms in the selected simple sequence loci.
  • Confirmed that these simple sequence length polymorphisms (SSLP) exhibit significant variability.

    • Conclusions:

    • Simple sequence length polymorphisms (SSLP) are a common and hypervariable feature of genomes.
    • SSLP can be effectively applied to identity testing, population studies, linkage analysis, and genome mapping.