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Updated: Mar 12, 2026

Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS
Published on: July 11, 2016
Routine identification of Nocardia species by MALDI-TOF mass spectrometry
Victoria Girard1, Sandrine Mailler1, Sophie Polsinelli1
1R&D Microbiology, bioMérieux, La Balme Les Grottes, France.
Abstract:
We here show adequate species identification for bacterial isolates of the genus Nocardia spp. through VITEK mass spectrometry. Application of a specific sample preparation method in combination with a robust matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) database leads to 94% accurate identification to the species level on a set of 164 isolates. The possibility to identify Nocardia spp. using MALDI-TOF MS will be available in the next release of VITEK MS update (IVD Version 3.0).
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