Library Quantification Using SYBR Green-Quantitative Polymerase Chain Reaction (qPCR)
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Updated: Mar 12, 2026

08:37
Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control
Published on: March 30, 2015
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Cold Spring Harbor Protocols
|November 3, 2016
Abstract:
To quantify complex DNA libraries, Kapa Biosystems engineered a DNA polymerase specifically for SYBR Green-based qPCR, enabling efficient amplification of targets such as GC-rich DNAs that present a challenge to wild-type DNA polymerases. Kapa Library Quantification Kits contain this engineered polymerase to ensure robust amplification of longer fragments, across a broad range of GC content.

