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Related Concept Videos

Real Time RT-PCR02:57

Real Time RT-PCR

66.1K
Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
66.1K

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Related Experiment Video

Updated: Mar 12, 2026

Quantification of dsDNA using the Hitachi F-7000 Fluorescence Spectrophotometer and PicoGreen Dye
07:44

Quantification of dsDNA using the Hitachi F-7000 Fluorescence Spectrophotometer and PicoGreen Dye

Published on: November 5, 2010

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Library Quantification: Fluorometric Quantitation of Double-Stranded or Single-Stranded DNA Samples Using the Qubit

Elaine Mardis, W Richard McCombie

    Cold Spring Harbor Protocols
    |November 3, 2016
    PubMed
    Summary
    This summary is machine-generated.

    The Qubit system offers accurate and sensitive fluorescence-based quantitation for nucleic acids. Optimized assay kits provide reliable detection ranges for both double-stranded DNA (dsDNA) and single-stranded DNA (ssDNA).

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    Simple Bulk Readout of Digital Nucleic Acid Quantification Assays
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    07:44

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    Simple Bulk Readout of Digital Nucleic Acid Quantification Assays
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    Simple Bulk Readout of Digital Nucleic Acid Quantification Assays

    Published on: September 24, 2015

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    Area of Science:

    • Biochemistry
    • Molecular Biology
    • Analytical Chemistry

    Background:

    • Accurate quantitation of nucleic acids is crucial for molecular biology applications.
    • Fluorescence-based methods offer high sensitivity and specificity for nucleic acid detection.
    • The Qubit fluorometer is a widely used system for nucleic acid quantitation.

    Purpose of the Study:

    • To evaluate the performance of Qubit assay kits for nucleic acid quantitation.
    • To determine the detection ranges of the high-sensitivity dsDNA Qubit Kit and the ssDNA Kit.
    • To assess the compatibility of Qubit assay kits with other fluorometers.

    Main Methods:

    • Utilized the Qubit fluorometer and optimized assay kits for nucleic acid quantitation.
    • Determined the detection range for double-stranded DNA (dsDNA) using the high-sensitivity dsDNA Qubit Kit.
    • Determined the detection range for single-stranded DNA (ssDNA) using the ssDNA Kit.

    Main Results:

    • The high-sensitivity dsDNA Qubit Kit demonstrated a detection range of 0.2-100 ng.
    • The ssDNA Kit exhibited a detection range of 1-200 ng.
    • Qubit assay kits function efficiently with the Qubit fluorometer and other fluorometers.

    Conclusions:

    • Qubit assay kits provide accurate and sensitive quantitation for both dsDNA and ssDNA.
    • The kits offer broad detection ranges suitable for various molecular biology applications.
    • The versatility of Qubit kits extends to compatibility with multiple fluorometer systems.