Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Designing studies for post-treatment Lyme disease and other infection-associated chronic illnesses.

Brain : a journal of neurology·2026

Same author

Patient-derived organoid xenografts reveal the multifaceted role of the lncRNA <i>MALAT1</i> in breast cancer progression.

bioRxiv : the preprint server for biology·2026

Same author

Integrative Genomic, Transcriptomic, and Microbiome Profiles of Colon Cancer by Ancestry Provide Insights into Molecular Distinctions.

medRxiv : the preprint server for health sciences·2026

Same author

Uncovering the early and conserved molecular mechanisms of root nitrogen foraging in model and crops.

BMC genomics·2026

Same author

Developmentally regulated genes drive phylogenomic splits in ovule evolution.

Nature communications·2025

Same author

Author Correction: MaizeCODE reveals bi-directionally expressed enhancers that harbor molecular signatures of maize domestication.

Nature communications·2025

Same journal

High-Throughput Microbial Assay for Amino Acid Measurement in Ground Maize Seed Samples Utilizing Auxotrophic <i>E. coli</i>.

Cold Spring Harbor protocols·2025

Same journal

Grain Quality in Maize.

Cold Spring Harbor protocols·2025

Same journal

High-Throughput Assay for Measuring Phytate and Available Phosphorus in Ground Maize Seed Samples.

Cold Spring Harbor protocols·2025

Same journal

Functional Genomic Analysis of Transposon Insertion Mutant Maize Plants from the UniformMu National Public Resource.

Cold Spring Harbor protocols·2025

Same journal

The UniformMu National Public Resource: Transposon<i>-</i>Induced Mutant Seeds for Functional Genomics Studies in Maize.

Cold Spring Harbor protocols·2025

Same journal

Insights from the Study of B<i>-</i>Cell Epitopes of a Microbial Pathogen by Phage Display.

Cold Spring Harbor protocols·2025

See all related articles

Search research articles

Related Experiment Video

Updated: Mar 12, 2026

Detecting Somatic Genetic Alterations in Tumor Specimens by Exon Capture and Massively Parallel Sequencing

Detecting Somatic Genetic Alterations in Tumor Specimens by Exon Capture and Massively Parallel Sequencing

Published on: October 18, 2013

Solution-Phase Exome Capture.

Elaine Mardis, W Richard McCombie

Cold Spring Harbor Protocols

|November 3, 2016

Summary

This summary is machine-generated.

This protocol details creating paired-end genomic DNA libraries for targeted sequencing. It outlines DNA capture methods and purification for reliable Illumina sequencing applications.

More Related Videos

A Novel Strategy Combining Array-CGH, Whole-exome Sequencing and In Utero Electroporation in Rodents to Identify Causative Genes for Brain Malformations

A Novel Strategy Combining Array-CGH, Whole-exome Sequencing and In Utero Electroporation in Rodents to Identify Causative Genes for Brain Malformations

Published on: December 1, 2017

Genome-wide Purification of Extrachromosomal Circular DNA from Eukaryotic Cells

Genome-wide Purification of Extrachromosomal Circular DNA from Eukaryotic Cells

Published on: April 4, 2016

Related Experiment Videos

Last Updated: Mar 12, 2026

Detecting Somatic Genetic Alterations in Tumor Specimens by Exon Capture and Massively Parallel Sequencing

Detecting Somatic Genetic Alterations in Tumor Specimens by Exon Capture and Massively Parallel Sequencing

Published on: October 18, 2013

A Novel Strategy Combining Array-CGH, Whole-exome Sequencing and In Utero Electroporation in Rodents to Identify Causative Genes for Brain Malformations

A Novel Strategy Combining Array-CGH, Whole-exome Sequencing and In Utero Electroporation in Rodents to Identify Causative Genes for Brain Malformations

Published on: December 1, 2017

Genome-wide Purification of Extrachromosomal Circular DNA from Eukaryotic Cells

Genome-wide Purification of Extrachromosomal Circular DNA from Eukaryotic Cells

Published on: April 4, 2016

Area of Science:

Genomics
Molecular Biology
Biotechnology

Background:

Genomic DNA (gDNA) sequencing requires efficient library preparation and targeted enrichment for specific genomic regions.
Next-generation sequencing (NGS) platforms necessitate high-quality DNA templates for accurate data generation.

Purpose of the Study:

To describe a comprehensive protocol for constructing paired-end genomic DNA libraries.
To detail the process of targeted DNA sequence capture using NimbleGen probes and Illumina TruSeq oligos.
To provide a method for purifying and quantifying captured DNA for downstream Illumina sequencing.

Main Methods:

Construction of paired-end libraries from genomic DNA (gDNA).
Sequence capture of specific genomic regions utilizing NimbleGen capture probes.
Ligation of Illumina TruSeq adapters for library preparation.
Purification and quantitation of captured DNA using magnetic AMPure bead cleanup.

Main Results:

The protocol yields purified and quantified DNA libraries suitable for Illumina sequencing.
The described methods ensure efficient capture of targeted genomic regions.
The AMPure bead cleanup procedure effectively removes contaminants and optimizes DNA quality.

Conclusions:

This protocol provides a robust method for preparing targeted genomic DNA libraries for NGS.
The described sequence capture and library construction approach is effective for various genomic applications.
The protocol ensures high-quality DNA templates, crucial for reliable and accurate sequencing results.