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Next-generation Sequencing

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....

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Related Experiment Video

Updated: Mar 12, 2026

Automated Gel Size Selection to Improve the Quality of Next-generation Sequencing Libraries Prepared from Environmental Water Samples

Automated Gel Size Selection to Improve the Quality of Next-generation Sequencing Libraries Prepared from Environmental Water Samples

Published on: April 17, 2015

Automated Library Preparation for DNA Sequencing.

Elaine Mardis, W Richard McCombie

Cold Spring Harbor Protocols

|November 3, 2016

Summary

This summary is machine-generated.

This study presents a generalized automated method for creating Illumina DNA libraries. This protocol simplifies the construction of both indexed and nonindexed libraries for research applications.

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Automated Robotic Liquid Handling Assembly of Modular DNA Devices

Automated Robotic Liquid Handling Assembly of Modular DNA Devices

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Ultra-long Read Sequencing for Whole Genomic DNA Analysis

Ultra-long Read Sequencing for Whole Genomic DNA Analysis

Published on: March 15, 2019

Related Experiment Videos

Last Updated: Mar 12, 2026

Automated Gel Size Selection to Improve the Quality of Next-generation Sequencing Libraries Prepared from Environmental Water Samples

Automated Gel Size Selection to Improve the Quality of Next-generation Sequencing Libraries Prepared from Environmental Water Samples

Published on: April 17, 2015

Automated Robotic Liquid Handling Assembly of Modular DNA Devices

Automated Robotic Liquid Handling Assembly of Modular DNA Devices

Published on: December 1, 2017

Ultra-long Read Sequencing for Whole Genomic DNA Analysis

Ultra-long Read Sequencing for Whole Genomic DNA Analysis

Published on: March 15, 2019

Area of Science:

Molecular Biology
Genomics
Biotechnology

Background:

Next-generation sequencing (NGS) relies on high-quality DNA libraries.
Manual library construction can be time-consuming and prone to errors.
Automation offers a solution for standardized and efficient library preparation.

Purpose of the Study:

To describe a generalized automated procedure for DNA library construction.
To enable the creation of both indexed and nonindexed Illumina DNA libraries.
To provide a reproducible and scalable method for researchers.

Main Methods:

Development of a generalized automated workflow.
Adaptation of existing library preparation steps for automation.
Validation of the automated procedure using Illumina sequencing platforms.

Main Results:

Successful construction of indexed and nonindexed Illumina DNA libraries.
Demonstration of reproducibility and consistency across multiple runs.
Quantification of library yields and sequencing performance.

Conclusions:

The automated procedure provides a robust and efficient method for DNA library preparation.
This protocol can accelerate genomic research by simplifying library construction.
The generalized approach is adaptable to various research needs and scales.