Library Quantification Using PicoGreen Fluorometry
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Updated: Mar 12, 2026
07:44
Quantification of dsDNA using the Hitachi F-7000 Fluorescence Spectrophotometer and PicoGreen Dye
Published on: November 5, 2010
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Cold Spring Harbor Protocols
|November 3, 2016
Abstract:
This protocol describes the quantification of DNA using PicoGreen and a fluorometer to determine the concentration of DNA sample for downstream processing. Because dye-based methods will not detect degraded or short DNA fragments, PicoGreen requires DNAs ≥50 bp, but can less reliably detect fragments as small as 20 bp.
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