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Related Experiment Video

Updated: Mar 12, 2026

Quantification of dsDNA using the Hitachi F-7000 Fluorescence Spectrophotometer and PicoGreen Dye

Quantification of dsDNA using the Hitachi F-7000 Fluorescence Spectrophotometer and PicoGreen Dye

Published on: November 5, 2010

Library Quantification Using PicoGreen Fluorometry.

Elaine Mardis, W Richard McCombie

Cold Spring Harbor Protocols

|November 3, 2016

Summary

This summary is machine-generated.

This protocol quantifies DNA concentration using PicoGreen dye and a fluorometer for reliable downstream processing. The method requires DNA fragments of at least 50 base pairs for accurate detection.

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Last Updated: Mar 12, 2026

Quantification of dsDNA using the Hitachi F-7000 Fluorescence Spectrophotometer and PicoGreen Dye

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Published on: November 5, 2010

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Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control

Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control

Published on: March 30, 2015

Area of Science:

Molecular Biology
Biochemistry
Analytical Chemistry

Background:

Accurate DNA quantification is crucial for molecular biology applications.
Traditional spectrophotometric methods can be influenced by contaminants.
Fluorescence-based assays offer increased sensitivity and specificity.

Purpose of the Study:

To describe a protocol for quantifying DNA concentration.
To utilize PicoGreen dye and a fluorometer for DNA measurement.
To ensure DNA sample suitability for downstream applications.

Main Methods:

DNA samples are incubated with PicoGreen dye.
Fluorescence intensity is measured using a fluorometer.
DNA concentration is determined based on fluorescence output.

Main Results:

The protocol provides a reliable method for DNA quantification.
PicoGreen accurately measures DNA concentrations for samples ≥50 bp.
Degraded or short DNA fragments (<50 bp) may be less reliably detected.

Conclusions:

This PicoGreen-based protocol is effective for DNA quantification.
The method is suitable for preparing DNA for downstream molecular assays.
Awareness of fragment size limitations is important for accurate results.