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Related Concept Videos

DNA Agarose Gel Electrophoresis02:35

DNA Agarose Gel Electrophoresis

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Agarose gel electrophoresis is a laboratory technique commonly used to separate DNA fragments by size. However, it can also be used to isolate and purify DNA fragments using a gel extraction protocol.
Gel extraction follows five major steps: running gel electrophoresis to separate fragments, isolating the individual bands, extracting DNA from those bands, and removing the dye and salts from the extracted mixture to obtain pure DNA.
In cloning experiments, both the insert and vector DNA...
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SDS-PAGE01:27

SDS-PAGE

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Gel electrophoresis is a method that separates biological macromolecules like nucleic acids or proteins by forcing them to pass through a gel matrix under an electric field.
A variation of gel electrophoresis, termed  polyacrylamide gel electrophoresis (PAGE), is commonly used for separating proteins according to their molecular size by passing them through a polyacrylamide gel. Because of the varying charges associated with amino acid side chains, PAGE can be used to separate intact...
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Southern Blot02:57

Southern Blot

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Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...
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Related Experiment Video

Updated: Mar 12, 2026

Automated Gel Size Selection to Improve the Quality of Next-generation Sequencing Libraries Prepared from Environmental Water Samples
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Agarose Gel Size Selection for DNA Sequencing Libraries.

Elaine Mardis, W Richard McCombie

    Cold Spring Harbor Protocols
    |November 3, 2016
    PubMed
    Summary
    This summary is machine-generated.

    Agarose gel electrophoresis purifies fragmented genomic DNA after adaptor ligation. DNA is extracted from excised gel bands and purified using a spin column for further analysis.

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    Area of Science:

    • Molecular Biology
    • Genomics
    • Biochemistry

    Background:

    • Fragmented genomic DNA often requires purification after molecular manipulations like adaptor ligation.
    • Efficient purification methods are crucial for downstream genomic applications.

    Purpose of the Study:

    • To describe a method for purifying fragmented genomic DNA using agarose gel electrophoresis.
    • To outline the steps involved in DNA extraction and purification post-electrophoresis.

    Main Methods:

    • Agarose gel electrophoresis is employed to separate DNA fragments by size.
    • Specific gel regions containing the desired DNA fragment sizes are physically excised.
    • DNA is extracted from the excised gel matrix.
    • Purification is achieved using a spin column chromatography method.

    Main Results:

    • Successful purification of fragmented genomic DNA based on size selection.
    • Obtained purified DNA suitable for subsequent molecular biology techniques.

    Conclusions:

    • Agarose gel electrophoresis followed by spin column purification is an effective strategy for obtaining size-selected fragmented genomic DNA.
    • This method provides purified DNA ready for downstream applications such as library preparation or sequencing.